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Effects of 30days bed rest and exercise countermeasures on PBMC bioenergetics

Büscher, F.-M. and Schmitz, M.T. and Frett, T. and Kramme, J. and de Boni, L. and Elmenhorst, E.-M. and Mulder, E. and Moestl, S. and Heusser, K. and Frings-Meuthen, P. and Jordan, J. and Rittweger, J. and Pesta, D. (2024) Effects of 30days bed rest and exercise countermeasures on PBMC bioenergetics. Acta Physiologica, 240 (3), e14102. Wiley. doi: 10.1111/apha.14102. ISSN 1748-1708.

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Official URL: https://doi.org/10.1111/apha.14102

Abstract

Abstract Aim: Altered mitochondrial function across various tissues is a key determinant of spaceflight-induced physical deconditioning. In comparison to tissue biopsies, blood cell bioenergetics holds promise as a systemic and more readily accessible biomarker, which was evaluated during head-down tilt bed rest (HDTBR), an established ground-based analog for spaceflight-induced physiological changes in humans. More specifically, this study explored the effects of HDTBR and an exercise countermeasure on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs). Methods: We subjected 24 healthy participants to a strict 30-day HDTBR protocol. The control group (n=12) underwent HDTBR only, while the countermeasure group (n=12) engaged in regular supine cycling exercise followed by veno-occlusive thigh cuffs post-exercise for 6h. We assessed routine blood parameters 14 days before bed rest, the respiratory capacity of PBMCs via highresolution respirometry, and citrate synthase activity 2days before and at day 30 of bed rest. We confirmed PBMC composition by flow cytometry. Results: The change of the PBMC maximal oxidative phosphorylation capacity (OXPHOS) amounted to an 11% increase in the countermeasure group, while it decreased by 10% in the control group (p=0.04). The limitation of OXPHOS increased in control only while other respiratory states were not affected by either intervention. Correlation analysis revealed positive associations between white blood cells, lymphocytes, and basophils with PBMC bioenergetics in both groups. Conclusion: This study reveals that a regular exercise countermeasure has a positive impact on PBMC mitochondrial function, confirming the potential application of blood cell bioenergetics for human spaceflight.

Item URL in elib:https://elib.dlr.de/203255/
Document Type:Article
Title:Effects of 30days bed rest and exercise countermeasures on PBMC bioenergetics
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Büscher, F.-M.German Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Schmitz, M.T.Institute of Medical Biometry, Informatics and Epidemiology (IMBIE), University Hospital Bonn, Bonn, GermanyUNSPECIFIEDUNSPECIFIED
Frett, T.UNSPECIFIEDhttps://orcid.org/0000-0002-5572-1177UNSPECIFIED
Kramme, J.German Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
de Boni, L.UNSPECIFIEDhttps://orcid.org/0000-0001-7785-482XUNSPECIFIED
Elmenhorst, E.-M.UNSPECIFIEDhttps://orcid.org/0000-0003-0336-6705UNSPECIFIED
Mulder, E.UNSPECIFIEDhttps://orcid.org/0000-0003-1200-5792UNSPECIFIED
Moestl, S.German Aerospace Center, Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Heusser, K.UNSPECIFIEDhttps://orcid.org/0000-0002-2571-5585UNSPECIFIED
Frings-Meuthen, P.UNSPECIFIEDhttps://orcid.org/0000-0001-5291-4419UNSPECIFIED
Jordan, J.UNSPECIFIEDhttps://orcid.org/0000-0003-4518-0706UNSPECIFIED
Rittweger, J.UNSPECIFIEDhttps://orcid.org/0000-0002-2223-8963UNSPECIFIED
Pesta, D.UNSPECIFIEDhttps://orcid.org/0000-0002-5089-3586UNSPECIFIED
Date:31 January 2024
Journal or Publication Title:Acta Physiologica
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:240
DOI:10.1111/apha.14102
Page Range:e14102
Publisher:Wiley
ISSN:1748-1708
Status:Published
Keywords:Blood cell bioenergetics, countermeasure, exercise, head-down tilt bed rest, mitochondrial function, PBMC, simulated microgravity, spaceflight
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Muscle Mechanics and Metabolism
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Institute of Aerospace Medicine > Leitungsbereich ME
Institute of Aerospace Medicine > Sleep and Human Factors Research
Institute of Aerospace Medicine > Office of Management and Budget
Deposited By: Kaiser-Stolz, Claudia
Deposited On:20 Mar 2024 09:25
Last Modified:26 Mar 2024 14:24

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