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The adhesion capability of Staphylococcus aureus cells is heterogeneously distributed over the cell envelope

Spengler, Christian and Maikranz, Erik and Glatz, Bernhard and Klatt, Michael Andreas and Heintz, Hannah and Bischoff, Markus and Santen, Ludger and Fery, Andreas and Jacobs, Karin (2024) The adhesion capability of Staphylococcus aureus cells is heterogeneously distributed over the cell envelope. Soft Matter, 20 (3), pp. 484-494. Royal Society of Chemistry. doi: 10.1039/D3SM01045G. ISSN 1744-683X.

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Official URL: https://dx.doi.org/10.1039/D3SM01045G

Abstract

Understanding and controlling microbial adhesion is a critical challenge in biomedical research, given the profound impact of bacterial infections on global health. Many facets of bacterial adhesion, including the distribution of adhesion forces across the cell wall, remain poorly understood. While a recent ‘patchy colloid’ model has shed light on adhesion in Gram-negative Escherichia coli cells, a corresponding model for Gram-positive cells has been elusive. In this study, we employ single cell force spectroscopy to investigate the adhesion force of Staphylococcus aureus. Normally, only one contact point of the entire bacterial surface is measured. However, by using a sine-shaped surface and recording force-distance curves along a path perpendicular to the rippled structures, we can characterize almost a hemisphere of one and the same bacterium. This unique approach allows us to study a greater number of contact points between the bacterium and the surface compared to conventional flat substrata. Distributed over the bacterial surface, we identify sites of higher and lower adhesion, which we call ‘patchy adhesion’, reminiscent of the patchy colloid model. The experimental results show that only some cells exhibit particularly strong adhesion at certain locations. To gain a better understanding of these locations, a geometric model of the bacterial cell surface was created. The experimental results were best reproduced by a model that features a few (5-6) particularly strong adhesion sites (diameter about 250 nm) that are widely distributed over the cell surface. Within the simulated patches, the number of molecules or their individual adhesive strength is increased. A more detailed comparison shows that simple geometric considerations for interacting molecules are not sufficient, but rather strong angle-dependent molecule-substratum interactions are required. We discuss the implications of our results for the development of new materials and the design and analysis of future studies.

Item URL in elib:https://elib.dlr.de/202658/
Document Type:Article
Title:The adhesion capability of Staphylococcus aureus cells is heterogeneously distributed over the cell envelope
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Spengler, ChristianUNSPECIFIEDhttps://orcid.org/0000-0002-0504-1149UNSPECIFIED
Maikranz, ErikUNSPECIFIEDhttps://orcid.org/0000-0001-6162-3605UNSPECIFIED
Glatz, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klatt, Michael AndreasUNSPECIFIEDhttps://orcid.org/0000-0002-1029-5960154571307
Heintz, HannahUNSPECIFIEDhttps://orcid.org/0009-0008-8400-176XUNSPECIFIED
Bischoff, MarkusUNSPECIFIEDhttps://orcid.org/0000-0001-6734-2732UNSPECIFIED
Santen, LudgerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fery, AndreasUNSPECIFIEDhttps://orcid.org/0000-0001-6692-3762UNSPECIFIED
Jacobs, KarinUNSPECIFIEDhttps://orcid.org/0000-0002-2963-2533UNSPECIFIED
Date:January 2024
Journal or Publication Title:Soft Matter
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:20
DOI:10.1039/D3SM01045G
Page Range:pp. 484-494
Publisher:Royal Society of Chemistry
ISSN:1744-683X
Status:Published
Keywords:Cell adhesion; stochastic geometry; atomic force microscope
HGF - Research field:other
HGF - Program:other
HGF - Program Themes:other
DLR - Research area:Digitalisation
DLR - Program:D - no assignment
DLR - Research theme (Project):D - no assignment
Location: Ulm
Institutes and Institutions:Institute for AI Safety and Security
Institute of Materials Physics in Space
Deposited By: Klatt, Dr. Michael Andreas
Deposited On:04 Mar 2024 08:17
Last Modified:04 Mar 2024 12:27

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