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Determination of spectral fluorescence cross sections of bacteria aerosols: Achievements, challenges, and limitations

Gruenewald, Karin and Hall, Thomas and Fellner, Lea and Walter, Arne and Kraus, Marian and Duschek, Frank (2023) Determination of spectral fluorescence cross sections of bacteria aerosols: Achievements, challenges, and limitations. SICC Series - CBRNe Conference, 2023-09-25 - 2023-09-29, Rom, Italien.

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Abstract

Spectral fluorescence cross sections of bacteria provide information on the distinguishability of bacterial species using fluorescence spectroscopy. A database of cross sections supports the evaluation of spectra achieved by optical detectors based on laser induced fluorescence. Furthermore, the dataset facilitates the selection of surrogates for pathogens in test environments by verifying similar fluorescent properties. The derivation of cross section data from measured fluorescence spectra necessitates the absolute calibration of the measuring system. In the presented device, the fluorescence is measured in a highly diluted aerosol flow of dry bacteria. The aerosol concentration is derived from microscopic analyses of samples extracted from the bacteria flow. Established calibration methods like Mie scattering at monodisperse spheres and Raman scattering of water are applied to the same set of fluorescence spectra. The achieved cross section data are evaluated with regard to their independency from the measuring system. The results are compared to referenced data. Discrepancies between different datasets in the literature are primarily attributed to different microbiological properties of bacteria due to the respective sample preparation, growth conditions, and aerosolization stress. Elusive optical influences on measurement and calibration as well as inaccuracies of the reference material cause further limitations for the comparability of datasets from different measuring systems.

Item URL in elib:https://elib.dlr.de/197799/
Document Type:Conference or Workshop Item (Speech)
Title:Determination of spectral fluorescence cross sections of bacteria aerosols: Achievements, challenges, and limitations
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Gruenewald, KarinUNSPECIFIEDhttps://orcid.org/0000-0003-0922-2307UNSPECIFIED
Hall, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fellner, LeaUNSPECIFIEDhttps://orcid.org/0000-0002-1359-0260UNSPECIFIED
Walter, ArneUNSPECIFIEDhttps://orcid.org/0000-0002-4377-9152UNSPECIFIED
Kraus, MarianUNSPECIFIEDhttps://orcid.org/0000-0002-5385-9420UNSPECIFIED
Duschek, FrankUNSPECIFIEDhttps://orcid.org/0000-0002-1809-0257UNSPECIFIED
Date:27 September 2023
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:bacteria aerosols, fluorescence cross sections, measurement and calibration
Event Title:SICC Series - CBRNe Conference
Event Location:Rom, Italien
Event Type:international Conference
Event Start Date:25 September 2023
Event End Date:29 September 2023
HGF - Research field:other
HGF - Program:other
HGF - Program Themes:other
DLR - Research area:Aeronautics
DLR - Program:L DT - Defense Technology
DLR - Research theme (Project):L - Support
Location: Lampoldshausen
Institutes and Institutions:Institute of Technical Physics > Atmospheric Propagation and Effect
Deposited By: Grünewald, Karin
Deposited On:13 Nov 2023 11:43
Last Modified:24 Apr 2024 20:58

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