Fellner, Lea and Kraus, Marian and Walter, Arne and Duschek, Frank and Bocklitz, Thomas and Gabbarini, Valentina and Rossi, Riccardo and Puleio, Alessandro and Malizia, Andrea and Gaudio, Pasquale (2021) Determination of composition of mixed biological samples using laser-induced fluorescence and combined classification/regression models. European Physical Journal Plus. Springer. doi: 10.1140/epjp/s13360-021-02019-1. ISSN 2190-5444.
![]() |
PDF
- Postprint version (accepted manuscript)
821kB |
![]() |
PDF
- Published version
815kB |
Abstract
Abstract Laser-induced fluorescence (LIF) provides the ability to distinguish organic materials by a fast and distant in situ analysis. When detecting the substances directly in the environment, e.g. in an aerosol cloud or on surfaces, additional fluorescence signals of other fluorophores occurring in the surrounding are expected to mix with the desired signal. We approached this problem with a simplified experimental design for an evaluation of classification algorithms. An upcoming question for enhanced identification capabilities is the case of mixed samples providing different signals from different fluorophores. For this work, mixtures of up to four common fluorophores (NADH, FAD, tryptophan, and tyrosine) were measured by a dual wavelength setup and spectrally analyzed. Classification and regression are conducted with neural networks and show an excellent performance in predicting the ratios of the selected ingredients.
Item URL in elib: | https://elib.dlr.de/139865/ | |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Document Type: | Article | |||||||||||||||||||||||||||||||||
Title: | Determination of composition of mixed biological samples using laser-induced fluorescence and combined classification/regression models | |||||||||||||||||||||||||||||||||
Authors: |
| |||||||||||||||||||||||||||||||||
Date: | 9 November 2021 | |||||||||||||||||||||||||||||||||
Journal or Publication Title: | European Physical Journal Plus | |||||||||||||||||||||||||||||||||
Refereed publication: | Yes | |||||||||||||||||||||||||||||||||
Open Access: | Yes | |||||||||||||||||||||||||||||||||
Gold Open Access: | No | |||||||||||||||||||||||||||||||||
In SCOPUS: | Yes | |||||||||||||||||||||||||||||||||
In ISI Web of Science: | Yes | |||||||||||||||||||||||||||||||||
DOI : | 10.1140/epjp/s13360-021-02019-1 | |||||||||||||||||||||||||||||||||
Editors: |
| |||||||||||||||||||||||||||||||||
Publisher: | Springer | |||||||||||||||||||||||||||||||||
ISSN: | 2190-5444 | |||||||||||||||||||||||||||||||||
Status: | Published | |||||||||||||||||||||||||||||||||
Keywords: | laser-induced fluorescence; LIF; classification and regression models; machine learning algorithms; neural networks; biological fluorophores; mixtures | |||||||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | |||||||||||||||||||||||||||||||||
HGF - Program: | Aeronautics | |||||||||||||||||||||||||||||||||
HGF - Program Themes: | Aircraft Research (old) | |||||||||||||||||||||||||||||||||
DLR - Research area: | Aeronautics | |||||||||||||||||||||||||||||||||
DLR - Program: | L AR - Aircraft Research | |||||||||||||||||||||||||||||||||
DLR - Research theme (Project): | L - Laser Research and Technology (old), L - Laser Research and Technology (old) | |||||||||||||||||||||||||||||||||
Location: | Lampoldshausen | |||||||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Technical Physics > Atmospheric Propagation and Effect Institute of Technical Physics | |||||||||||||||||||||||||||||||||
Deposited By: | Kraus, Marian | |||||||||||||||||||||||||||||||||
Deposited On: | 13 Jan 2021 12:06 | |||||||||||||||||||||||||||||||||
Last Modified: | 17 Nov 2021 16:25 |
Repository Staff Only: item control page