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Fluorescence Spectra of a Bacterial Population During Different Growth Phases

Fellner, Lea and Gebert, Florian and Walter, Arne and Grünewald, Karin and Duschek, Frank (2017) Fluorescence Spectra of a Bacterial Population During Different Growth Phases. In: Springer. 1st Scientific International Conference on CBRNE, 22.-24.Mai 2017, Rom, Italien.

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Abstract

Biological agents like pathogenic bacteria represent a major threat to the public if spread. Bacteria may replicate in their host and can spread in an unpredictable way. Standoff detection based on laser induced fluorescence may help to mitigate the associated risks. Nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and tryptophan are among others the most important fluorophores in bacteria. Bacteria adapt to their environment and thus the same bacterial species may be composed of different components and relative concentrations of them depending on environmental conditions. Fluorescence spectra of a Bacillus thuringiensis population were compared during different growth phases. Laser pulses with two different excitation wavelengths, 280 nm and 355 nm, were used. For 280 nm excitation the measured spectra show a difference in spectral features between the bacterial population before sporulation and after it was partially sporulated. In comparison a smaller variation in 355 nm excited LIF spectra of a bacterial population during exponential growth and the ageing population is observed.

Item URL in elib:https://elib.dlr.de/114401/
Document Type:Conference or Workshop Item (Poster)
Title:Fluorescence Spectra of a Bacterial Population During Different Growth Phases
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fellner, Lealea.fellner (at) dlr.dehttps://orcid.org/0000-0002-1359-0260
Gebert, Florianflorian.gebert (at) dlr.deUNSPECIFIED
Walter, Arnearne.walter (at) dlr.dehttps://orcid.org/0000-0002-4377-9152
Grünewald, Karinkarin.gruenewald (at) dlr.dehttps://orcid.org/0000-0003-0922-2307
Duschek, Frankfrank.duschek (at) dlr.dehttps://orcid.org/0000-0002-1809-0257
Date:22 May 2017
Journal or Publication Title:Springer
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Laser induced fluorescence, Bacteria, Spores, Growth phases, Spectroscopy
Event Title:1st Scientific International Conference on CBRNE
Event Location:Rom, Italien
Event Type:international Conference
Event Dates:22.-24.Mai 2017
Organizer:University of Rome Tor Vergata
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Aeronautics
HGF - Program Themes:fixed-wing aircraft
DLR - Research area:Aeronautics
DLR - Program:L AR - Aircraft Research
DLR - Research theme (Project):L - Laser Research and Technology
Location: Lampoldshausen
Institutes and Institutions:Institute of Technical Physics > Atmospheric Propagation and Effect
Deposited By: Fellner, Lea
Deposited On:28 Nov 2017 10:56
Last Modified:24 Jan 2020 11:12

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