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Standoff detection and classification of bacteria by multispectral laser-induced fluorescence

Duschek, Frank and Fellner, Lea and Gebert, Florian and Grünewald, Karin and Köhntopp, Anja and Kraus, Marian and Mahnke, Peter and Pargmann, Carsten and Tomaso, Herbert and Walter, Arne (2017) Standoff detection and classification of bacteria by multispectral laser-induced fluorescence. Advanced Optical Technologies, 6 (2), pp. 75-83. de Gruyter. DOI: 10.1515/aot-2016-0066 ISBN 2192-8576 (ISSN) ISSN 2192-8576

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Official URL: https://www.degruyter.com/view/j/aot.2017.6.issue-2/aot-2016-0066/aot-2016-0066.xml?format=INT

Abstract

Biological hazardous substances such as certain fungi and bacteria represent a high risk for the broad public if fallen into wrong hands. Incidents based on bio-agents are commonly considered to have unpredictable and complex consequences for first responders and people. The impact of such an event can be minimized by an early and fast detection of hazards. The presented approach is based on optical standoff detection applying laser-induced fluorescence (LIF) on bacteria. The LIF bio-detector has been designed for outdoor operation at standoff distances from 20 m up to more than 100 m. The detector acquires LIF spectral data for two different excitation wavelengths (280 and 355 nm) which can be used to classify suspicious samples. A correlation analysis and spectral classification by a decision tree is used to discriminate between the measured samples. In order to demonstrate the capabilities of the system, suspensions of the low-risk and non-pathogenic bacteria Bacillus thuringiensis, Bacillus atrophaeus, Bacillus subtilis, Brevibacillus brevis, Micrococcus luteus, Oligella urethralis, Paenibacillus polymyxa and Escherichia coli (K12) have been investigated with the system, resulting in a discrimination accuracy of about 90%.

Item URL in elib:https://elib.dlr.de/114111/
Document Type:Article
Title:Standoff detection and classification of bacteria by multispectral laser-induced fluorescence
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Duschek, Frankfrank.duschek (at) dlr.dehttps://orcid.org/0000-0002-1809-0257
Fellner, Lealea.fellner (at) dlr.dehttps://orcid.org/0000-0002-1359-0260
Gebert, Florianflorian.gebert (at) dlr.deUNSPECIFIED
Grünewald, Karinkarin.gruenewald (at) dlr.dehttps://orcid.org/0000-0003-0922-2307
Köhntopp, AnjaAnja.Koehntopp (at) dlr.deUNSPECIFIED
Kraus, MarianMarian.Kraus (at) dlr.deUNSPECIFIED
Mahnke, Peterpeter.mahnke (at) dlr.deUNSPECIFIED
Pargmann, CarstenCarsten.Pargmann (at) dlr.dehttps://orcid.org/0000-0003-3688-6360
Tomaso, Herbertfriedrich-loeffler-institut, institute of bacterial infections and zoonoses, naumburger strasse 96a, 07743 jenaUNSPECIFIED
Walter, Arnearne.walter (at) dlr.dehttps://orcid.org/0000-0002-4377-9152
Date:April 2017
Journal or Publication Title:Advanced Optical Technologies
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:6
DOI :10.1515/aot-2016-0066
Page Range:pp. 75-83
Publisher:de Gruyter
ISSN:2192-8576
ISBN:2192-8576 (ISSN)
Status:Published
Keywords:bacteria; biological agents; classification; laser-induced fluorescence (LIF); standoff detection
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Aeronautics
HGF - Program Themes:fixed-wing aircraft
DLR - Research area:Aeronautics
DLR - Program:L AR - Aircraft Research
DLR - Research theme (Project):L - Laser Research and Technology
Location: Lampoldshausen
Institutes and Institutions:Institute of Technical Physics > Atmospheric Propagation and Effect
Deposited By: Kraus, Marian
Deposited On:28 Nov 2017 10:55
Last Modified:14 Dec 2019 04:24

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