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The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuaeto Simulated Outer Space Solar Radiation

Leuko, S. and Domingos, C. and Parpart, A. and Reitz, G. and Rettberg, P. (2015) The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuaeto Simulated Outer Space Solar Radiation. Astrobiology, 15 (11), pp. 987-997. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2015.1310 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2015.1310

Abstract

Solar radiation is among the most prominent stress factors organisms face during space travel and possibly on other planets. Our analysis of three different halophilic archaea, namely Halobacterium salinarum NRC-1, Halococcus morrhuae, and Halococcus hamelinensis, which were exposed to simulated solar radiation in either dried or liquid state, showed tremendous differences in tolerance and survivability. We found that Hcc. hamelinensis is not able to withstand high fluences of simulated solar radiation compared to the other tested organisms. These results can be correlated to significant differences in genomic integrity following exposure, as visualized by random amplified polymorphic DNA (RAPD)–PCR. In contrast to the other two tested strains, Hcc. hamelinensis accumulates compatible solutes such as trehalose for osmoprotection. The addition of 100mM trehalose to the growth medium of Hcc. hamelinensis improved its survivability following exposure. Exposure of cells in liquid at different temperatures suggests that Hbt. salinarum NRC-1 is actively repairing cellular and DNA damage during exposure, whereas Hcc. morrhuae exhibits no difference in survival. For Hcc. morrhuae, the high resistance against simulated solar radiation may be explained with the formation of cell clusters. Our experiments showed that these clusters shield cells on the inside against simulated solar radiation, which results in better survival rates at higher fluences when compared to Hbt. salinarum NRC-1 and Hcc. hamelinensis. Overall, this study shows that some halophilic archaea are highly resistant to simulated solar radiation and that they are of high astrobiological significance.

Item URL in elib:https://elib.dlr.de/99297/
Document Type:Article
Title:The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuaeto Simulated Outer Space Solar Radiation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Leuko, S.Deutsches Zentrum für Luft- und Raumfahrt, Institut für Luft- und Raumfahrtmedizin, Abteilung Strahlenbiologie, Arbeitsgruppe Astrobiologie, Köln, Germany.UNSPECIFIED
Domingos, C.Faculty of Sciences, University of Lisbon, Lisbon, Portugal.UNSPECIFIED
Parpart, A.Deutsches Zentrum für Luft- und Raumfahrt, Institut für Luft- und Raumfahrtmedizin, Abteilung Strahlenbiologie, Köln, Germany.UNSPECIFIED
Reitz, G.Deutsches Zentrum für Luft- und Raumfahrt, Institut für Luft- und Raumfahrtmedizin, Abteilung Strahlenbiologie, Köln, Germany.UNSPECIFIED
Rettberg, P.Deutsches Zentrum für Luft- und Raumfahrt, Institut für Luft- und Raumfahrtmedizin, Abteilung Strahlenbiologie, Arbeitsgruppe Astrobiologie, Köln, Germany.UNSPECIFIED
Date:2015
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:15
DOI :10.1089/ast.2015.1310
Page Range:pp. 987-997
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Halophiles, Solar radiation, Stress resistance, Survival
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:10 Nov 2015 15:20
Last Modified:06 Sep 2019 15:23

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