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Confocal Raman microspectroscopy reveals a convergence of the chemical composition in methanogenic archaea from a Siberian permafrost-affected soil

Serrano, P. and Hermelink, A. and Lasch, P. and de Vera, Jean Pierre Paul and König, N. and Burckhardt, O. and Wagner, D. (2015) Confocal Raman microspectroscopy reveals a convergence of the chemical composition in methanogenic archaea from a Siberian permafrost-affected soil. Fems Microbiology Ecology. Federation of European Microbiological Societies, Published by Blackwell Publishing Ltd.. DOI: 10.1093/femsec/fiv126 ISSN 0168-6496

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Abstract

Methanogenic archaea are widespread anaerobic microorganisms responsible for the 25 production of biogenic methane. Several new species of psychrotolerant methanogenic archaea were recently isolated from a permafrost-affected soil in the Lena delta (Siberia, Russia), showing an exceptional resistance against desiccation, osmotic stress, low temperatures, starvation, UV and ionizing radiation when compared to methanogens from non-permafrost environments. To gain a deeper insight into the differences observed in their resistance, we described the chemical composition of methanogenic strains from permafrost and non-permafrost environments using confocal Raman microspectroscopy (CRM). CRM is a powerful tool for microbial identification and provides fingerprint-like information about 33 the chemical composition of the cells. Our results show that the chemical composition of methanogens from permafrost-affected soils presents a high homology and is remarkably different from strains inhabiting non-permafrost environments. In addition, we performed a phylogenetic reconstruction of the studied strains based on the functional gene mcrA to prove the different evolutionary relationship of the permafrost strains. We conclude that the permafrost methanogenic strains show a convergent chemical composition regardless of their genotype. This fact is likely to be the consequence of a complex adaptive process to the Siberian permafrost environment and might be the reason underlying their resistant nature.

Item URL in elib:https://elib.dlr.de/98843/
Document Type:Article
Title:Confocal Raman microspectroscopy reveals a convergence of the chemical composition in methanogenic archaea from a Siberian permafrost-affected soil
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Serrano, P.Geoforschungszentrum, PotsdamUNSPECIFIED
Hermelink, A.Robert Koch Institute. Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
Lasch, P.Robert Koch Institute, Centre of biological Safety 6; Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
König, N.Robert Koch Institute, Centre of biological Safety 6; Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
Burckhardt, O.Geoforschungszentrum, PotsdamUNSPECIFIED
Wagner, D.Geoforschungszentrum, PotsdamUNSPECIFIED
Date:23 October 2015
Journal or Publication Title:Fems Microbiology Ecology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1093/femsec/fiv126
Publisher:Federation of European Microbiological Societies, Published by Blackwell Publishing Ltd.
ISSN:0168-6496
Status:Published
Keywords:methanogenic archaea; Siberian permafrost; confocal Raman microspectroscopy; chemical composition; environmental adaptations; mcrA
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:17 Nov 2015 13:50
Last Modified:06 Sep 2019 15:19

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