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Metabarcoding of the kombucha microbial community grown in different microenvironments

Reva, O.M. and Zaets, I. and Ovcharenko, L.P. and Kukharenko, O. and Shpylova, S.P. and Podolich, O.V. and de Vera, Jean Pierre Paul and Kozyrovska, N. (2015) Metabarcoding of the kombucha microbial community grown in different microenvironments. AMB Express. Springer. DOI: 10.1186/s13568-015-0124-5 ISSN 2191-0855

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Official URL: http://link.springer.com/article/10.1186%2Fs13568-015-0124-5#

Abstract

Introducing of the DNA metabarcoding analysis of probiotic microbial communities allowed getting insight into their functioning and establishing a better control on safety and efficacy of the probiotic communities. In this work the kombucha poly-microbial probiotic community was analysed to study its flexibility under different growth conditions. Environmental DNA sequencing revealed a complex and flexible composition of the kombucha microbial culture (KMC) constituting more bacterial and fungal organisms in addition to those found by cultural method. The community comprised bacterial and yeast components including cultured and uncultivable microorganisms. Culturing the KMC under different conditions revealed the core part of the community which included acetobacteria of two genera Komagataeibacter (former Gluconacetobacter) and Gluconobacter, and representatives of several yeast genera among which Brettanomyces/Dekkera and Pichia (including former Issatchenkia) were dominant. Herbaspirillum spp. and Halomonas spp., which previously had not been described in KMC, were found to be minor but permanent members of the community. The community composition was dependent on the growth conditions. The bacterial component of KMC was relatively stable, but may include additional member—lactobacilli. The yeast species composition was significantly variable. High-throughput sequencing showed complexity and variability of KMC that may affect the quality of the probiotic drink. It was hypothesized that the kombucha core community might recruit some environmental bacteria, particularly lactobacilli, which potentially may contribute to the fermentative capacity of the probiotic drink. As many KMC-associated microorganisms cannot be cultured out of the community, a robust control for community composition should be provided by using DNA metabarcoding.

Item URL in elib:https://elib.dlr.de/98842/
Document Type:Article
Title:Metabarcoding of the kombucha microbial community grown in different microenvironments
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Reva, O.M.Department of Biochemistry, Bioinformatics and Computational Biology Unit, University of Pretoria, South AfricaUNSPECIFIED
Zaets, I.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
Ovcharenko, L.P.Institute of Molecular Biology & Genetics of National Academy of Sciences of UkraineUNSPECIFIED
Kukharenko, O.Institute of Molecular Biology & Genetics of National Academy of Sciences of UkraineUNSPECIFIED
Shpylova, S.P.Institute of Molecular Biology & Genetics of National Academy of Sciences of UkraineUNSPECIFIED
Podolich, O.V.Institute of Molecular Biology & Genetics of National Academy of Sciences of UkraineUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Kozyrovska, N.Institute of Molecular Biology&Genetics of National Academy of Sciences, Kyiv, UkraineUNSPECIFIED
Date:11 June 2015
Journal or Publication Title:AMB Express
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1186/s13568-015-0124-5
Publisher:Springer
ISSN:2191-0855
Status:Published
Keywords:Kombucha microbial community; Metabarcoding; Pyrosequencing
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:17 Nov 2015 13:47
Last Modified:01 Dec 2018 19:51

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