elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments

Nagler, Katja and Setlow, Peter and Reineke, Kai and Driks, Adam and Moeller, Ralf (2015) Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments. Applied and Environmental Microbiology, 81 (19), pp. 6725-6735. American Society for Microbiology. DOI: 10.1128/AEM.01817-15 ISSN 0099-2240

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1128/AEM.01817-15

Abstract

The germination of spore-forming bacteria in high-salinity environments is of applied interest for food microbiology and soil ecology. It has previously been shown that high salt concentrations detrimentally affect Bacillus subtilis spore germination, rendering this process slower and less efficient. The mechanistic details of these salt effects, however, remained obscure. Since initiation of nutrient germination first requires germinant passage through the spores’ protective integuments, the aim of this study was to elucidate the role of the proteinaceous spore coat in germination in high-salinity environments. Spores lacking major layers of the coat due to chemical decoating or mutation germinated much worse in the presence of NaCl than untreated wild-type spores at comparable salinities. However, the absence of the crust, the absence of some individual nonmorphogenetic proteins, and the absence of either CwlJ or SleB had no or little effect on germination in high-salinity environments. Although the germination of spores lacking GerP (which is assumed to facilitate germinant flow through the coat) was generally less efficient than the germination of wild-type spores, the presence of up to 2.4MNaCl enhanced the germination of these mutant spores. Interestingly, nutrient-independent germination by high pressure was also inhibited by NaCl. Taken together, these results suggest that (i) the coat has a protective function during germination in high-salinity environments; (ii) germination inhibition by NaCl is probably not exerted at the level of cortex hydrolysis, germinant accessibility, or germinant-receptor binding; and (iii) the most likely germination processes to be inhibited by NaCl are ion, Ca²⁺-dipicolinic acid, and water fluxes.

Item URL in elib:https://elib.dlr.de/98135/
Document Type:Article
Title:Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Nagler, KatjaGerman Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyUNSPECIFIED
Setlow, PeterDepartment of Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USAUNSPECIFIED
Reineke, KaiLeibnitz Institute for Agricultural Engineering, Department of Horticultural Engineering, Potsdam, GermanyUNSPECIFIED
Driks, AdamStritch School of Medicine, Loyola University Chicago, Department of Microbiology and Immunology, Maywood, Illinois, USAUNSPECIFIED
Moeller, RalfGerman Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyUNSPECIFIED
Date:2015
Journal or Publication Title:Applied and Environmental Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:81
DOI :10.1128/AEM.01817-15
Page Range:pp. 6725-6735
Publisher:American Society for Microbiology
ISSN:0099-2240
Status:Published
Keywords:Bacillus subtilis, high-salinity environments, spore germination, salt stress, salt-dependent germination
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:14 Sep 2015 09:39
Last Modified:14 Sep 2015 09:39

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.