elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Datenschutz | Kontakt | English
Schriftgröße: [-] Text [+]

Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments

Nagler, Katja und Setlow, Peter und Reineke, Kai und Driks, Adam und Moeller, Ralf (2015) Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments. Applied and Environmental Microbiology, 81 (19), Seiten 6725-6735. American Society for Microbiology. doi: 10.1128/AEM.01817-15. ISSN 0099-2240.

Dieses Archiv kann nicht den Volltext zur Verfügung stellen.

Offizielle URL: http://dx.doi.org/10.1128/AEM.01817-15

Kurzfassung

The germination of spore-forming bacteria in high-salinity environments is of applied interest for food microbiology and soil ecology. It has previously been shown that high salt concentrations detrimentally affect Bacillus subtilis spore germination, rendering this process slower and less efficient. The mechanistic details of these salt effects, however, remained obscure. Since initiation of nutrient germination first requires germinant passage through the spores’ protective integuments, the aim of this study was to elucidate the role of the proteinaceous spore coat in germination in high-salinity environments. Spores lacking major layers of the coat due to chemical decoating or mutation germinated much worse in the presence of NaCl than untreated wild-type spores at comparable salinities. However, the absence of the crust, the absence of some individual nonmorphogenetic proteins, and the absence of either CwlJ or SleB had no or little effect on germination in high-salinity environments. Although the germination of spores lacking GerP (which is assumed to facilitate germinant flow through the coat) was generally less efficient than the germination of wild-type spores, the presence of up to 2.4MNaCl enhanced the germination of these mutant spores. Interestingly, nutrient-independent germination by high pressure was also inhibited by NaCl. Taken together, these results suggest that (i) the coat has a protective function during germination in high-salinity environments; (ii) germination inhibition by NaCl is probably not exerted at the level of cortex hydrolysis, germinant accessibility, or germinant-receptor binding; and (iii) the most likely germination processes to be inhibited by NaCl are ion, Ca²⁺-dipicolinic acid, and water fluxes.

elib-URL des Eintrags:https://elib.dlr.de/98135/
Dokumentart:Zeitschriftenbeitrag
Titel:Involvement of Coat Proteins in Bacillus subtilis Spore Germination in High-Salinity Environments
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Nagler, KatjaGerman Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Setlow, PeterDepartment of Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USANICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Reineke, KaiLeibnitz Institute for Agricultural Engineering, Department of Horticultural Engineering, Potsdam, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Driks, AdamStritch School of Medicine, Loyola University Chicago, Department of Microbiology and Immunology, Maywood, Illinois, USANICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, RalfGerman Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Datum:2015
Erschienen in:Applied and Environmental Microbiology
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:81
DOI:10.1128/AEM.01817-15
Seitenbereich:Seiten 6725-6735
Verlag:American Society for Microbiology
ISSN:0099-2240
Status:veröffentlicht
Stichwörter:Bacillus subtilis, high-salinity environments, spore germination, salt stress, salt-dependent germination
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:14 Sep 2015 09:39
Letzte Änderung:20 Nov 2023 14:24

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Gestaltung Webseite und Datenbank: Copyright © Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.