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Resistance of the Lichen Buellia frigidato Simulated Space Conditions during the Preflight Tests for BIOMEX—Viability Assay and Morphological Stability

Meeßen, J. and Wuthenow, P. and Schille, P. and Rabbow, E. and de Vera, J.-P.P. and Ott, S. (2015) Resistance of the Lichen Buellia frigidato Simulated Space Conditions during the Preflight Tests for BIOMEX—Viability Assay and Morphological Stability. Astrobiology, 15 (8), pp. 601-615. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2015.1281 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2015.1281

Abstract

Samples of the extremotolerant Antarctic endemite lichen Buellia frigida are currently exposed to low-Earth orbit– space and simulated Mars conditions at the Biology and Mars Experiment (BIOMEX), which is part of the ESA mission EXPOSE-R2 on the International Space Station and was launched on 23 July 2014. In preparation for the mission, several preflight tests (Experimental and Scientific Verification Tests, EVT and SVT) assessed the sample preparation and hardware integration procedures as well as the resistance of the candidate organism toward the abiotic stressors experienced under space and Mars conditions. Therefore, we quantified the post-exposure viability with a live/dead staining technique utilizing FUN-1 and confocal laser scanning microscopy (CLSM). In addition, we used scanning electron microscopy (SEM) to investigate putative patterns of morphological-anatomical damage that lichens may suffer under the extreme exposure conditions. The present results demonstrate that Buellia frigida is capable of surviving the conditions tested in EVT and SVT. The mycobiont showed lower average impairment of its viability than the photobiont (viability rates of >83% and >69%, respectively), and the lichen thallus suffered no significant damage in terms of thalline integrity and symbiotic contact. These results will become essential to substantiate and validate the results prospectively obtained from the returning space mission. Moreover, they will help assess the limits and limitations of terrestrial organisms under space and Mars conditions as well as characterize the adaptive traits that confer lichen extremotolerance.

Item URL in elib:https://elib.dlr.de/97916/
Document Type:Article
Title:Resistance of the Lichen Buellia frigidato Simulated Space Conditions during the Preflight Tests for BIOMEX—Viability Assay and Morphological Stability
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Meeßen, J.Institut für Botanik, Heinrich-Heine Universität (HHU), Düsseldorf, Germany.UNSPECIFIED
Wuthenow, P.Institut für Botanik, Heinrich-Heine Universität (HHU), Düsseldorf, Germany.UNSPECIFIED
Schille, P.Institut für Botanik, Heinrich-Heine Universität (HHU), Düsseldorf, Germany.UNSPECIFIED
Rabbow, E.Institut für Luft- und Raumfahrtmedizin, Deutsches Zentrum für Luft- und Raumfahrt (DLR), Köln, Germany.UNSPECIFIED
de Vera, J.-P.P.Institut für Planetenforschung, Deutsches Zentrum für Luft- und Raumfahrt (DLR), Berlin, Germany.UNSPECIFIED
Ott, S.Institut für Botanik, Heinrich-Heine Universität (HHU), Düsseldorf, Germany.UNSPECIFIED
Date:2015
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:15
DOI :10.1089/ast.2015.1281
Page Range:pp. 601-615
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Astrobiology, BIOMEX, EXPOSE-R2, Extremotolerance, Lichens
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Planetary Research > Experimentelle Planetenphysik
Deposited By: Kopp, Kerstin
Deposited On:14 Sep 2015 09:37
Last Modified:06 Sep 2019 15:23

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