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Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers

Wehland, Markus and Aleshcheva, Ganna and Schulz, Herbert and Saar, Katrin and Hübner, Norbert and Hemmersbach, Ruth and Braun, Markus and Ma, Xiao and Frett, Timo and Warnke, Elisabeth and Riwaldt, Stefan and Pietsch, Jessica and Corydon, Thomas Juhl and Infanger, Manfred and Grimm, Daniela (2015) Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers. Cell Communication and Signaling, pp. 1-13. BioMed Central. DOI: 10.1186/s12964-015-0095-9 ISSN 1478-811X

Full text not available from this repository.

Official URL: http://www.biosignaling.com/content/13/1/18

Abstract

Background: Chondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain, has only very limited potential to heal. One future possibility to replace damaged cartilage might be the scaffold-free growth of chondrocytes in microgravity to form 3D aggregates. Results: To prepare for this, we have conducted experiments during the 20th DLR parabolic flight campaign, where we fixed the cells after the first (1P) and the 31st parabola (31P). Furthermore, we subjected chondrocytes to isolated vibration and hypergravity conditions. Microarray and quantitative real time PCR analyses revealed that hypergravity regulated genes connected to cartilage integrity (BMP4, MMP3, MMP10, EDN1, WNT5A, BIRC3). Vibration was clearly detrimental to cartilage (upregulated inflammatory IL6 and IL8, downregulated growth factors EGF, VEGF, FGF17). The viability of the cells was not affected by the parabolic flight, but showed a significantly increased expression of anti-apoptotic genes after 31 parabolas. The IL-6 release of chondrocytes cultured under conditions of vibration was not changed, but hypergravity (1.8 g) induced a clear elevation of IL-6 protein in the supernatant compared with corresponding control samples. Conclusion: Taken together, this study provided new insights into the growth behavior of chondrocytes under short-term microgravity.

Item URL in elib:https://elib.dlr.de/96717/
Document Type:Article
Title:Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Wehland, MarkusClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, Magdeburg, 39120, GermanyUNSPECIFIED
Aleshcheva, GannaSpaceLife, DLR Institute of Aerospace Medicine, Biomedical Research, Cologne, Germany and University of MagdeburgUNSPECIFIED
Schulz, HerbertMax-Delbrück-Center for Molecular Medicine, Berlin, 13092, Germany and University of Cologne, Cologne Center for GenomicsUNSPECIFIED
Saar, KatrinMax-Delbrück-Center for Molecular Medicine, Berlin, 13092, GermanyUNSPECIFIED
Hübner, NorbertMax-Delbruck-Center for Molecular Medicine, Berlin-Buch, GermanyUNSPECIFIED
Hemmersbach, RuthDLR German Aerospace Center, Biomedical Research, Gravitational Biology, Köln, 51147, GermanyUNSPECIFIED
Braun, MarkusGravitational Biology, IMBIO, University of Bonn, GermanyUNSPECIFIED
Ma, XiaoDepartment of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, Aarhus C, DK-8000, DenmarkUNSPECIFIED
Frett, TimoGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Research, Köln, 51147, GermanyUNSPECIFIED
Warnke, ElisabethClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Riwaldt, StefanClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Pietsch, JessicaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Corydon, Thomas JuhlDepartment of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, Aarhus C, DK-8000, DenmarkUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaDepartment of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, Aarhus C, DK-8000, DenmarkUNSPECIFIED
Date:20 March 2015
Journal or Publication Title:Cell Communication and Signaling
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1186/s12964-015-0095-9
Page Range:pp. 1-13
Publisher:BioMed Central
ISSN:1478-811X
Status:Published
Keywords:Chondrocytes, Gene expression, Microgravity, Hypergravity, Vibration, Cytokines
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:30 Jun 2015 10:23
Last Modified:21 Sep 2019 05:06

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