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Whey protein supplementation attenuates the reduction in muscle oxidative capacity during 21 days bed rest

Bosutti, Alessandra and Blottner, Dieter and Salanova, Michele and Rittweger, Jörn and Degens, Hans (2015) Whey protein supplementation attenuates the reduction in muscle oxidative capacity during 21 days bed rest. In: Journal of Muscle Research and Cell Motility, 36, pp. 112-113. Springer International Publishing Switzerland 2015. European Muscle Conference 2014, 10- 14 September 2014, Salzburg, Austria. DOI: 10.1007/s10974-015-9407-3

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Abstract

Muscle unloading, as encountered during space flight and disuse, induces skeletal muscle atrophy and weakness, switch of fibre types and loss of capillaries. Less attention has been given to disuse-induced changes in oxidative capacity, which may affect endurance. Here we investigated the impact of 21 days, 60 head-down bed rest on muscle fibre size, fibre type composition, capillarisation and muscle oxidative capacity and the efficacy of milk-based proteins (whey protein) to counteract disuse-induced muscle changes. Muscle biopsies were taken from the soleus (SOL) and vastus lateralis (VL)muscles before and after bed rest from10 healthy men (aged 31 ± 6 years). Subjects were assigned to two groups and studied twice in two follow-up campaigns, in once receiving whey protein and once receiving a standardized isocaloric diet. Fibre types and capillaries were identified by immunohistochemical staining of muscle section with anti-myosin type1 and lectin. Analysis was done with the method of capillary domains.Oxidative capacity of individual fibres was determined as the optical density (OD) at 660 nm of succinate dehydrogenase (SDH) stained histological sections. The fibre cross-sectional area times the SDH-OD of that fibre (integrated SDH) was index of maximal oxygen consumption of that fibre(VO2max). Statistical analysis was done by repeated measures ANOVA. Bed rest did not induce significant changes infibre type composition, fibre size or capillarisation, but decreased SDH activity (p\0.001) and fibre VO2max (p\0.01) in both muscles. Whey protein partially counteracted the decrease in oxidative capacity. Our results suggest that decrements in oxidative capacity may be an early hallmark of disuse-induced muscle adaptations and occurs even in the absence of significant fibre atrophy and capillary rarefaction. The Whey diet attenuated these effects and may thus be an effective supplement to sustain oxidative capacity during space flight and long-term bed rest.

Item URL in elib:https://elib.dlr.de/95752/
Document Type:Conference or Workshop Item (Speech)
Title:Whey protein supplementation attenuates the reduction in muscle oxidative capacity during 21 days bed rest
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Bosutti, AlessandraDepartment of Clinical, Technological and Morphological Sciences, Division of Internal Medicine, University Triest, ItalienUNSPECIFIED
Blottner, DieterCharité - University Medicine BerlinUNSPECIFIED
Salanova, MicheleCharité - University Medicine BerlinUNSPECIFIED
Rittweger, Jörnjoern.rittweger (at) dlr.deUNSPECIFIED
Degens, HansManchester Metropolitan UniversityUNSPECIFIED
Date:2015
Journal or Publication Title:Journal of Muscle Research and Cell Motility
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:36
DOI :10.1007/s10974-015-9407-3
Page Range:pp. 112-113
Publisher:Springer International Publishing Switzerland 2015
Status:Published
Keywords:Bed rest, Muscle disuse, Muslce oxidative capacity
Event Title:European Muscle Conference 2014
Event Location:Salzburg, Austria
Event Type:international Conference
Event Dates:10- 14 September 2014
Organizer:University of Salzburg
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Becker, Christine
Deposited On:26 Mar 2015 16:20
Last Modified:26 Mar 2015 16:20

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