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Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by up-regulation of BMP-2 and SOX-9.

Aleshcheva, Ganna and Wehland, Markus and Jayashree, Sahana and Bauer, Johann and Corydon, Thomas and Hemmersbach, Ruth and Frett, Timo and Egli, Marcel and Infanger, Manfred and Grimm, Daniela (2015) Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by up-regulation of BMP-2 and SOX-9. FASEB JOURNAL. Federation of American Societies for Experimental Biology. DOI: 10.1096/fj.14-268151 ISSN 0892-6638

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Abstract

Real and simulated microgravity induce a variety of changes in human cells. Most importantly, changes in the cytoskeleton have been noted, and studies on microtubules have shown that they are gravi-sensitive. This study focuses on the effects of short-term real microgravity on gene expression, protein content, and cytoskeletal structure of human chondrocytes. We cultivated human chondrocytes, took them along a parabolic flight during the 24th Deutsches Zentrum für Luft- und Raumfahrt Parabolic (DLR) Flight Campaign, and fixed them after the 1st and the 31st parabola. Immunofluorescence microscopy revealed no changes after the 1st parabola, but disruptions of β-tubulin, vimentin, and cytokeratin networks after the 31st parabola. No F-actin stress fibers were detected even after 31 parabolas. Furthermore, mRNA and protein quantifications after the 31st parabola showed a clear up-regulation of cytoskeletal genes and proteins. The mRNAs were significantly up-regulated as follows: TUBB, 2-fold; VIM, 1.3-fold; KRT8, 1.8-fold; ACTB, 1.9-fold; ICAM1, 4.8-fold; OPN, 7-fold; ITGA10, 1.5-fold; ITGB1, 1.2-fold; TGFB1, 1.5-fold; CAV1, 2.6-fold; SOX9, 1.7-fold; BMP-2, 5.3-fold. However, SOX5 (−25%) and SOX6 (−28%) gene expression was decreased. Contrary, no significant changes in gene expression levels were observed during vibration and hypergravity experiments. These data suggest that short-term microgravity affects the gene expression of distinct proteins. In contrast to poorly differentiated follicular thyroid cancer cells or human endothelial cells, chondrocytes only exert moderate cytoskeletal alterations. The up-regulation of BMP-2, TGF-β1, and SOX9 in chondrocytes may play a key role in preventing cytoskeletal alterations.—Aleshcheva, G., Wehland, M., Sahana, J., Bauer, J., Corydon, T. J., Hemmersbach, R., Frett, T., Egli, M., Infanger, M., Grosse, J., and Grimm, D. Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by up-regulation of BMP-2 and SOX-9.

Item URL in elib:https://elib.dlr.de/95468/
Document Type:Article
Title:Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by up-regulation of BMP-2 and SOX-9.
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Aleshcheva, GannaOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Jayashree, SahanaInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Corydon, ThomasInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Hemmersbach, RuthDLR German Aerospace Center, Biomedical Research, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Frett, TimoDLR German Aerospace Center, Biomedical Research, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Egli, MarcelSpace Biology Group, ETH Zurich, Zurich, SwitzerlandUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, DenmarkUNSPECIFIED
Date:13 February 2015
Journal or Publication Title:FASEB JOURNAL
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1096/fj.14-268151
Publisher:Federation of American Societies for Experimental Biology
ISSN:0892-6638
Status:Published
Keywords:F-actin; gene expression; hypergravity; vibration
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Frett, Timo
Deposited On:03 Mar 2015 11:40
Last Modified:08 Mar 2018 18:27

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