elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Life Sciences in Space Research: Constitutive Expression of tdTomato Protein as a Cytotoxicity and Proliferation Marker for Space Radiation Biology

Chishti, Arif A. and Hellweg, Christine E. and Berger, Thomas and Baumstark-Khan, Christa and Feles, Sebastian and Kätzel, Thorben and Reitz, Günther (2014) Life Sciences in Space Research: Constitutive Expression of tdTomato Protein as a Cytotoxicity and Proliferation Marker for Space Radiation Biology. Life Sciences in Space Research, 4, pp. 35-45. Elsevier. DOI: 10.1016/j.lssr.2014.12.005 ISSN 2214-5524

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.lssr.2014.12.005

Abstract

The radiation risk assessment for long-term space missions requires knowledge on the biological effectiveness of different space radiation components, e.g. heavy ions, on the interaction of radiation and other space environmental factors such as microgravity, and on the physical and biological dose distribution in the human body. Space experiments and ground-based experiments at heavy ion accelerators require fast and reliable test systems with an easy readout for different endpoints. In order to determine the effect of different radiation qualities on cellular proliferation and the biological depth dose distribution after heavy ion exposure, a stable human cell line expressing a novel fluorescent protein was established and characterized. tdTomato, a red fluorescent protein of the new generation with fast maturation and high fluorescence intensity, was selected as reporter of cell proliferation. Human embryonic kidney (HEK/293) cells were stably transfected with a plasmid encoding tdTomato under the control of the constitutively active cytomegalovirus (CMV) promoter (ptdTomato-N1). The stably transfected cell line was named HEK-ptdTomato-N1 8. This cytotoxicity biosensor was tested by ionizing radiation (X-rays and accelerated heavy ions) exposure. As biological endpoints, the proliferation kinetics and the cell density reached 100 h after irradiation reflected by constitutive expression of the tdTomato were investigated. Both were reduced dose-dependently after radiation exposure. Finally, the cell line was used for biological weighting of heavy ions of different linear energy transfer (LET) as space-relevant radiation quality. The relative biological effectiveness of accelerated heavy ions in reducing cellular proliferation peaked at an LET of 91 keV/μm. The results of this study demonstrate that the HEK-ptdTomato-N1 reporter cell line can be used as a fast and reliable biosensor system for detection of cytotoxic damage caused by ionizing radiation.

Item URL in elib:https://elib.dlr.de/94807/
Document Type:Article
Title:Life Sciences in Space Research: Constitutive Expression of tdTomato Protein as a Cytotoxicity and Proliferation Marker for Space Radiation Biology
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Chishti, Arif A.German Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, Germany; arif.chishti (at) dlr.deUNSPECIFIED
Hellweg, Christine E.German Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, GermanyUNSPECIFIED
Berger, ThomasGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, GermanyUNSPECIFIED
Baumstark-Khan, ChristaGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, GermanyUNSPECIFIED
Feles, SebastianGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, GermanyUNSPECIFIED
Kätzel, ThorbenGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, Germany and Present Address: Institut für Humangenetik, Biomedizinisches Zentrum, Universitätsklinikum Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn.UNSPECIFIED
Reitz, GüntherGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Linder Höhe, D-51147 Köln, GermanyUNSPECIFIED
Date:2014
Journal or Publication Title:Life Sciences in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:No
Volume:4
DOI :10.1016/j.lssr.2014.12.005
Page Range:pp. 35-45
Publisher:Elsevier
ISSN:2214-5524
Status:Published
Keywords:Mammalian cells, Radiation response,Fluorescent protein, Cytotoxicity, Proliferation, tdTomato
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:29 Jan 2015 11:37
Last Modified:29 Jan 2015 11:37

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.