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Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest

Blottner, D and Bosutti, Alessandra and Degens, Hans and Schiffl, G and Gutsmann, M and Bühlmeier, Judith and Rittweger, Jörn and Ganse, Bergita and Heer, M. and Salanova, M (2014) Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest. Journal of Musculoskeletal and Neuronal Interactions, 14 (4), pp. 432-444. International Society of Musculoskeletal and Neuronal Interactions. ISSN 1108-7161

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Abstract

Objectives: To investigate the effect of whey protein plus potassium bicarbonate supplement on disused skeletal muscle structure and proteolysis after bed rest (BR). Methods: Soleus (SOL) and vastus lateralis (VL) biopsies were sampled from ten (n=10) healthy male subjects (aged 31±6 years) who did BR once with and once without protein supplement as a dietary countermeasure (cross-over study design). The structural changes (myofibre size and type distribution) were analysed by histological sections, and muscle protein breakdown indirectly via the proteolysis markers, calpain 1 and 3, calpastatin, MuRF1 and 2, both in muscle homogenates and by immunohistochemistry. Results: BR caused size-changes in myofiber cross-sectional area (FCSA, SOL, p=0,004; VL, p=0.03), and myofiber slow-to-fast type transition with increased hybrids (SOL, p=0.043; VL, p=0.037) however with campaign differences in SOL (p<0.033). No significant effect of BR and supplement was found by any of the key proteolysis markers. Conclusions: Campaign differences in structural muscle adaptation may be an issue in cross-over design BR studies. The whey protein plus potassium bicarbonate supplement did not attenuate atrophy and fibre type transition during medium term bed rest. Alkaline whey protein supplements may however be beneficial as adjuncts to exercise countermeasures in disuse.

Item URL in elib:https://elib.dlr.de/94693/
Document Type:Article
Title:Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Blottner, DCharité Universitätsmedizin BerlinUNSPECIFIED
Bosutti, AlessandraDepartment of Clinical, Technological and Morphological Sciences, Division of Internal Medicine, University Triest, ItalienUNSPECIFIED
Degens, HansManchester Metropolitan UniversityUNSPECIFIED
Schiffl, GCenter of Space Medicine Berlin (ZWMB), BerlinUNSPECIFIED
Gutsmann, MCharité Universitätsmedizin Berlin,UNSPECIFIED
Bühlmeier, JudithUniversity of Bonn, GermanyUNSPECIFIED
Rittweger, JörnInstitute of Aerospace Medicine, Deutsches Zentrum für Luft- und Raumfahrt, DLR e.V., Cologne, Germany and Medical Faculty, University of Cologne, Cologne, Germany; joern.rittweger (at) dlr.deUNSPECIFIED
Ganse, BergitaDivision Space Physiology, Institute of Aerospace Medicine, German Aerospace Center, Cologne, bergita.ganse (at) dlr.deUNSPECIFIED
Heer, M.Institut für Ernährungswissenschaft, Universität Bonn, GermanyUNSPECIFIED
Salanova, MCenter of Space Medicine Berlin (ZWMB)UNSPECIFIED
Date:12 September 2014
Journal or Publication Title:Journal of Musculoskeletal and Neuronal Interactions
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:14
Page Range:pp. 432-444
Publisher:International Society of Musculoskeletal and Neuronal Interactions
ISSN:1108-7161
Status:Published
Keywords:Bed Rest, Whey Protein, Disuse, Muscle Atrophy, Proteolysis Biomarkers
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Becker, Christine
Deposited On:20 Jan 2015 10:09
Last Modified:10 Jan 2019 15:51

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