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The Role of Purinergic Signaling in Endothelial and Smooth Muscle Cells under Simulated Microgravity

Zhang, Yu and Lau, Patrick and Pansky, Andreas and Kassack, Matthias and Hemmersbach, Ruth and Tobiasch, Edda (2014) The Role of Purinergic Signaling in Endothelial and Smooth Muscle Cells under Simulated Microgravity. In: Purinergic Signalling, 10 (4). Springer. Purines International Conference on Nucleotides Nucleosides and Nucleobases International Conference on Signalling, Drugs and Targets, 23. - 27. Juli 2014, Bonn, Germany. ISSN 1573-9538

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Official URL: http://link.springer.com/article/10.1007/s11302-014-9430-7

Abstract

Astronauts suffer from cardiovascular deconditioning during space flight where they are exposed to microgravity. Alterations under real and simulated microgravity have been found e.g. in the cytoskeleton and apoptosis in endothelial cells (ECs) and smooth muscle cells (SMCs)1, 2. P2 receptors play an important role in a variety of vascular functions of ECs and SMCs. However, the functional role of purinergic signalling in ECs and SMCs under microgravity is still unclear. In this study primary ECs and SMCs were isolated from bovine aorta and characterized using specific markers. Additionally, EC growth medium collected during culture under normal gravity was used as conditioned medium for SMCs and vice versa to mimic a co-culture model. Here we show for the first time that the P2-receptor expression pattern is altered in ECs and SMCs under simulated microgravity achieved by a clinostat. Interestingly, conditioned medium compensated the alterations in the expression of specific P2-receptors. P2X7 was down-regulated in ECs after 24h clinorotation but recovered to the gene and protein expression level found under normal gravity when cultured in conditioned medium from SMCs. Our results showed an altered P2-receptor expression pattern under simulated microgravity. The paracrine effect between ECs and SMCs seems to be an important regulator of cell behaviour under altered gravity conditions. Several artificial P2-receptor ligands are already utilized as drugs. Thus it might be reasonable to consider them for drug development for astronaut treatment of cardiovascular deconditioning in the future.

Item URL in elib:https://elib.dlr.de/94509/
Document Type:Conference or Workshop Item (Speech)
Title:The Role of Purinergic Signaling in Endothelial and Smooth Muscle Cells under Simulated Microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Zhang, YuDLR Institute of Aerospace Medicine, SpaceLife, Biomedical Research, Cologne, GermanyUNSPECIFIED
Lau, PatrickGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Space Physiology, Cologne, GermanyUNSPECIFIED
Pansky, AndreasHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Kassack, MatthiasUniversität DüsseldorfUNSPECIFIED
Hemmersbach, RuthDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Tobiasch, EddaHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Date:3 December 2014
Journal or Publication Title:Purinergic Signalling
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:10
Publisher:Springer
ISSN:1573-9538
Status:Published
Keywords:purinergic signaling, endothelial cells, smooth muscle cells, simulated microgravity, P2X7
Event Title:Purines International Conference on Nucleotides Nucleosides and Nucleobases International Conference on Signalling, Drugs and Targets
Event Location:Bonn, Germany
Event Type:international Conference
Event Dates:23. - 27. Juli 2014
Organizer:University of Bonn
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:21 Jan 2015 10:36
Last Modified:21 Jan 2015 10:36

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