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The Impact of Simulated Microgravity on Purinergic Signaling in an Endothelial and Smooth Muscle Cell Co-Culture Model

Zhang, Yu and Lau, Patrick and Pansky, Andreas and Kassack, Matthias and Hemmersbach, Ruth and Tobiasch, Edda (2014) The Impact of Simulated Microgravity on Purinergic Signaling in an Endothelial and Smooth Muscle Cell Co-Culture Model. In: 40th COSPAR Scientific Assembly 2014. 40th COSPAR Scientific Assembly 2014, 2. - 10. August 2014, Moskau, Russland.

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Official URL: https://www.cospar-assembly.org/admin/session_cospar.php?session=510

Abstract

Astronauts suffer from cardiovascular deconditioning when they are exposed to microgravity conditions during space missions. Thus, current research focuses on the identification of the underlying mechanism also with respect to therapy and countermeasures. Endothelial cells (ECs) and smooth muscle cells (SMCs) play a key role in a variety of vascular functions. Gene expression, cytoskeleton morphology and apoptosis in both, ECs and SMCs, have shown alterations under simulated and real microgravity condition. However, all these data were observed during single culturing of either ECs or SMCs under microgravity conditions, which is different from the in vivo situation. Purinergic 2 (P2) receptors bind extracellular nucleotides and can regulate the vascular tone and vascular cell proliferation, migration and apoptosis. In this study primary ECs and SMCs were obtained from bovine aorta and characterized using specific markers. Here we show for the first time that the P2-receptor expressions pattern in ECs and in SMCs is altered after 24h in simulated microgravity. Specific receptors are down- or up-regulated on the gene and protein level. In addition the supernatant of ECs during culture was used as conditioned medium for SMCs and vice visa to investigate the influence of either cell type on the other. ECs and SMCs secret cytokines which induce pathogenic proliferation and an altered migration behavior under simulated microgravity conditions. Interestingly, co-culturing with condition medium could compensate this change. In detail, P2X7 was down-regulated in ECs after 24h clinorotation but recovered to the 1 g level when cultured with conditioned medium from SMCs collected under normal gravity. In conclusion, our data indicate that the paracrine effect between ECs and SMCs is an important regulator of cell behavior, also under altered gravity conditions. P2-receptor gene and protein expression were altered during microgravity. Since several P2-receptor artificial ligands are already established as drugs, P2- receptors might be a reasonable candidate for drug development for astronaut treatment of vascular deconditioning in the future.

Item URL in elib:https://elib.dlr.de/94505/
Document Type:Conference or Workshop Item (Speech)
Title:The Impact of Simulated Microgravity on Purinergic Signaling in an Endothelial and Smooth Muscle Cell Co-Culture Model
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Zhang, YuDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Lau, PatrickDLR Institute of Aerospace Medicine, Space Physiology, Cologne, GermanyUNSPECIFIED
Pansky, AndreasHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Kassack, MatthiasUniversität DüsseldorfUNSPECIFIED
Hemmersbach, RuthDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Tobiasch, EddaHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Date:5 August 2014
Journal or Publication Title:40th COSPAR Scientific Assembly 2014
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:purinergic signaling, endothelial cells, smooth muscle cells, co-culture, clinostat, simulated microgravity
Event Title:40th COSPAR Scientific Assembly 2014
Event Location:Moskau, Russland
Event Type:international Conference
Event Dates:2. - 10. August 2014
Organizer:Lomonosov Moscow State University
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:21 Jan 2015 10:32
Last Modified:21 Jan 2015 10:32

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