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Changes in Gene Expression, Protein Content and Morphology of Chondrocytes Cultured on a 3D Random Position Machine and 2D Rotating Clinostat

Aleshcheva, Ganna and Hauslage, Jens and Hemmersbach, Ruth and Infanger, Manfred and Bauer, Johann and Grimm, Daniela and Sahana, Jayashree (2014) Changes in Gene Expression, Protein Content and Morphology of Chondrocytes Cultured on a 3D Random Position Machine and 2D Rotating Clinostat. In: 40th COSPAR Scientific Assembly. 40th COSPAR Scientific Assemby 2014, 2. - 10. August 2014, Moskau, Russland.

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Abstract

Chondrocytes are the only cell type found in human cartilage consisting of proteoglycans and type II collagen. Several studies on chondrocytes cultured either in Space or on a ground-based facility for simulation of microgravity revealed that these cells are very resistant to adverse effects and stress induced by altered gravity. Tissue engineering of chondrocytes is a new strategy for cartilage regeneration. Using a three-dimensional Random Positioning Machine and a 2D rotating clinostat, devices designed to simulate microgravity on Earth, we investigated the early effects of microgravity exposure on human chondrocytes of six different donors after 30 min, 2 h, 4 h, 16 h, and 24 h and compared the results with the corresponding static controls cultured under normal gravity conditions. As little as 30 min of exposure resulted in increased expression of several genes responsible for cell motility, structure and integrity (beta-actin); control of cell growth, cell proliferation, cell differentiation and apoptosis; and cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours disruptions in the vimentin network were detected. These changes were less dramatic after 16 hours, when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-1 was enhanced for 24 h. Based on the results achieved, we suggest that chondrocytes exposed to simulated microgravity seem to change their extracellular matrix production behavior while they rearrange their cytoskeletal proteins prior to forming three-dimensional aggregates.

Item URL in elib:https://elib.dlr.de/94356/
Document Type:Conference or Workshop Item (Speech)
Title:Changes in Gene Expression, Protein Content and Morphology of Chondrocytes Cultured on a 3D Random Position Machine and 2D Rotating Clinostat
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Aleshcheva, GannaSpacelife, Otto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Hauslage, JensDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, DenmarkUNSPECIFIED
Sahana, JayashreeInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Date:5 August 2014
Journal or Publication Title:40th COSPAR Scientific Assembly
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:gene expression, chondrocytes, simulated microgravity, random positioning machine, rotating clinostat
Event Title:40th COSPAR Scientific Assemby 2014
Event Location:Moskau, Russland
Event Type:international Conference
Event Dates:2. - 10. August 2014
Organizer:Lomonosov Moscow State University
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:08 Jan 2015 15:48
Last Modified:08 Jan 2015 15:48

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