Serrano, P. and Hermelink, A. and Lasch, P. and de Vera, Jean Pierre Paul and Böttger, Ute and Wagner, D. (2014) Biosignatures of methanogenic archaea by Confocal Raman Microspectroscopy (CRM). EANA 2014, 13.-16. Okt. 2014, Edinburgh, UK.
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Official URL: http://www.astrobiology.ac.uk/files/2013/03/Abstract-Book-EANA.pdf
Abstract
Methanogenic archaea are anaerobic chemotrophic microorganisms that meet many of the metabolic and physiological requirements for survival on the martian subsurface. In particular, methanogens from Siberian permafrost are extremely resistant against different types of environmental stresses as well as simulated martian thermo-physical and subsurface conditions, making them promising model organisms for potential life on Mars. Raman spectroscopy is a vibrational spectroscopic technique that has shown a remarkable potential in microbial identification. It provides fingerprint-like information about the overall chemical composition of the samples and allows a nondestructive investigation. The biosignatures of Methanosarcina soligelidi SMA-21 were characterized by CRM during the growth phases at a single-cell level, which presented a high heterogeneity and diversity in the chemical composition of the cells and detectible subpopulation differences. This study also highlighted potential technical challenges concerning the Raman detection of methanogenic archaea (and other non-pigmented microorganisms) embedded on a mineral substrate. The biosignatures of permafrost and non-permafrost strains in the stationary phase of growth were also characterized by CRM. A cluster analysis of the spectra revealed that permafrost and non-permafrost strains have a different overall chemical composition, which has possible evolutionary implications.
Item URL in elib: | https://elib.dlr.de/94150/ | |||||||||||||||||||||
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Document Type: | Conference or Workshop Item (Speech) | |||||||||||||||||||||
Title: | Biosignatures of methanogenic archaea by Confocal Raman Microspectroscopy (CRM) | |||||||||||||||||||||
Authors: |
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Date: | 13 October 2014 | |||||||||||||||||||||
Refereed publication: | No | |||||||||||||||||||||
Open Access: | Yes | |||||||||||||||||||||
Gold Open Access: | No | |||||||||||||||||||||
In SCOPUS: | No | |||||||||||||||||||||
In ISI Web of Science: | No | |||||||||||||||||||||
Status: | Published | |||||||||||||||||||||
Keywords: | methanogenic archaea; CRM; biosignatures; Mars relevance; | |||||||||||||||||||||
Event Title: | EANA 2014 | |||||||||||||||||||||
Event Location: | Edinburgh, UK | |||||||||||||||||||||
Event Type: | international Conference | |||||||||||||||||||||
Event Dates: | 13.-16. Okt. 2014 | |||||||||||||||||||||
Organizer: | UK Centre of Astrobiology | |||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | |||||||||||||||||||||
HGF - Program: | Space | |||||||||||||||||||||
HGF - Program Themes: | Space Science and Exploration | |||||||||||||||||||||
DLR - Research area: | Raumfahrt | |||||||||||||||||||||
DLR - Program: | R EW - Erforschung des Weltraums | |||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Exploration des Sonnensystems | |||||||||||||||||||||
Location: | Berlin-Adlershof | |||||||||||||||||||||
Institutes and Institutions: | Institute of Planetary Research > Experimentelle Planetenphysik | |||||||||||||||||||||
Deposited By: | de Vera, Dr. Jean Pierre Paul | |||||||||||||||||||||
Deposited On: | 06 Jan 2015 14:51 | |||||||||||||||||||||
Last Modified: | 31 Jul 2019 19:51 |
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