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Biosignatures of methanogenic archaea by Confocal Raman Microspectroscopy (CRM)

Serrano, P. and Hermelink, A. and Lasch, P. and de Vera, Jean Pierre Paul and Böttger, Ute and Wagner, D. (2014) Biosignatures of methanogenic archaea by Confocal Raman Microspectroscopy (CRM). EANA 2014, 13.-16. Okt. 2014, Edinburgh, UK.

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Official URL: http://www.astrobiology.ac.uk/files/2013/03/Abstract-Book-EANA.pdf


Methanogenic archaea are anaerobic chemotrophic microorganisms that meet many of the metabolic and physiological requirements for survival on the martian subsurface. In particular, methanogens from Siberian permafrost are extremely resistant against different types of environmental stresses as well as simulated martian thermo-physical and subsurface conditions, making them promising model organisms for potential life on Mars. Raman spectroscopy is a vibrational spectroscopic technique that has shown a remarkable potential in microbial identification. It provides fingerprint-like information about the overall chemical composition of the samples and allows a nondestructive investigation. The biosignatures of Methanosarcina soligelidi SMA-21 were characterized by CRM during the growth phases at a single-cell level, which presented a high heterogeneity and diversity in the chemical composition of the cells and detectible subpopulation differences. This study also highlighted potential technical challenges concerning the Raman detection of methanogenic archaea (and other non-pigmented microorganisms) embedded on a mineral substrate. The biosignatures of permafrost and non-permafrost strains in the stationary phase of growth were also characterized by CRM. A cluster analysis of the spectra revealed that permafrost and non-permafrost strains have a different overall chemical composition, which has possible evolutionary implications.

Item URL in elib:https://elib.dlr.de/94150/
Document Type:Conference or Workshop Item (Speech)
Title:Biosignatures of methanogenic archaea by Confocal Raman Microspectroscopy (CRM)
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Serrano, P.Geoforschungszentrum, PotsdamUNSPECIFIED
Hermelink, A.Robert Koch Institute. Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
Lasch, P.Robert Koch Institute, Centre of biological Safety 6; Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Böttger, UteUte.Boettger (at) dlr.deUNSPECIFIED
Wagner, D.Geoforschungszentrum, PotsdamUNSPECIFIED
Date:13 October 2014
Refereed publication:No
Open Access:Yes
Gold Open Access:No
In ISI Web of Science:No
Keywords:methanogenic archaea; CRM; biosignatures; Mars relevance;
Event Title:EANA 2014
Event Location:Edinburgh, UK
Event Type:international Conference
Event Dates:13.-16. Okt. 2014
Organizer:UK Centre of Astrobiology
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Experimentelle Planetenphysik
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:06 Jan 2015 14:51
Last Modified:31 Jul 2019 19:51

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