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Survival and viability of cells from iron depositing bacterial strains in pretests for the EXPOSE-R2-Experiment

Feyh, N. and de Vera, Jean Pierre Paul and Szewzyk, U. (2014) Survival and viability of cells from iron depositing bacterial strains in pretests for the EXPOSE-R2-Experiment. EANA 2014, 13.-16. Okt. 2014, Edinburgh, UK.

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Official URL: http://www.astrobiology.ac.uk/files/2013/03/Abstract-Book-EANA.pdf


Five environmental isolates (Pseudomonas sp. BS1, Hyphomonas sp. BS2, Tetrasphaera sp. FL1, Pedomicrobium sp. FL6 and Leptothrix sp. OT_B_406) were chosen for EXPOSE-R2 including pretests (EVT1/2, SVT) due to their ability to form Fe(III)-oxyhydroxide-containing biofilms as observed for natural communities of iron depositing bacteria. Samples were produced by drying iron-containing cell aggregates on Mars regolith simulant mixtures (S-/P-MRS) (Böttger et al., 2012). Different Mars- and Space-relevant treatments were applied during EVT1/2 and SVT: artificial atmosphere, vacuum, UV-C irradiation and temperatures from -25 °C to 60 °C. Viability of cells was determined afterwards by recultivation and Fluorescence in-situ hybridization (FISH). In order to estimate membrane integrity, cell staining was applied as standalone-method and in combination with subsequent qPCR (PMA-qPCR) (Nocker et al., 2008). Strains FL1 and FL6 were found to be recultivable from most of the samples. FISH-positive cells were detected in all sample types. PMA-qPCR was found to be superior to classic LIVE-DEAD staining due to being more sensitive. Culture independent techniques like FISH and qPCR can help to make more precise estimates about survival of microorganisms by detecting viable but not culturable cells.

Item URL in elib:https://elib.dlr.de/94146/
Document Type:Conference or Workshop Item (Poster)
Title:Survival and viability of cells from iron depositing bacterial strains in pretests for the EXPOSE-R2-Experiment
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Feyh, N.Institut für Umwelttechnologie, TU BerlinUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Szewzyk, U.Institut für Umwelttechnologie, TU BerlinUNSPECIFIED
Date:13 October 2014
Refereed publication:No
Open Access:Yes
Gold Open Access:No
In ISI Web of Science:No
Keywords:iron bacteria; EXPOSE-R2; space experiments; survival; viability
Event Title:EANA 2014
Event Location:Edinburgh, UK
Event Type:international Conference
Event Dates:13.-16. Okt. 2014
Organizer:UK Centre of Astrobiology
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Experimentelle Planetenphysik
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:06 Jan 2015 14:44
Last Modified:31 Jul 2019 19:51

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