Feyh, N. und de Vera, Jean Pierre Paul und Szewzyk, U. (2014) Survival and viability of cells from iron depositing bacterial strains in pretests for the EXPOSE-R2-Experiment. EANA 2014, 2014-10-13 - 2014-10-16, Edinburgh, UK.
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Offizielle URL: http://www.astrobiology.ac.uk/files/2013/03/Abstract-Book-EANA.pdf
Kurzfassung
Five environmental isolates (Pseudomonas sp. BS1, Hyphomonas sp. BS2, Tetrasphaera sp. FL1, Pedomicrobium sp. FL6 and Leptothrix sp. OT_B_406) were chosen for EXPOSE-R2 including pretests (EVT1/2, SVT) due to their ability to form Fe(III)-oxyhydroxide-containing biofilms as observed for natural communities of iron depositing bacteria. Samples were produced by drying iron-containing cell aggregates on Mars regolith simulant mixtures (S-/P-MRS) (Böttger et al., 2012). Different Mars- and Space-relevant treatments were applied during EVT1/2 and SVT: artificial atmosphere, vacuum, UV-C irradiation and temperatures from -25 °C to 60 °C. Viability of cells was determined afterwards by recultivation and Fluorescence in-situ hybridization (FISH). In order to estimate membrane integrity, cell staining was applied as standalone-method and in combination with subsequent qPCR (PMA-qPCR) (Nocker et al., 2008). Strains FL1 and FL6 were found to be recultivable from most of the samples. FISH-positive cells were detected in all sample types. PMA-qPCR was found to be superior to classic LIVE-DEAD staining due to being more sensitive. Culture independent techniques like FISH and qPCR can help to make more precise estimates about survival of microorganisms by detecting viable but not culturable cells.
elib-URL des Eintrags: | https://elib.dlr.de/94146/ | ||||||||||||||||
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Dokumentart: | Konferenzbeitrag (Poster) | ||||||||||||||||
Titel: | Survival and viability of cells from iron depositing bacterial strains in pretests for the EXPOSE-R2-Experiment | ||||||||||||||||
Autoren: |
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Datum: | 13 Oktober 2014 | ||||||||||||||||
Referierte Publikation: | Nein | ||||||||||||||||
Open Access: | Ja | ||||||||||||||||
Gold Open Access: | Nein | ||||||||||||||||
In SCOPUS: | Nein | ||||||||||||||||
In ISI Web of Science: | Nein | ||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||
Stichwörter: | iron bacteria; EXPOSE-R2; space experiments; survival; viability | ||||||||||||||||
Veranstaltungstitel: | EANA 2014 | ||||||||||||||||
Veranstaltungsort: | Edinburgh, UK | ||||||||||||||||
Veranstaltungsart: | internationale Konferenz | ||||||||||||||||
Veranstaltungsbeginn: | 13 Oktober 2014 | ||||||||||||||||
Veranstaltungsende: | 16 Oktober 2014 | ||||||||||||||||
Veranstalter : | UK Centre of Astrobiology | ||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||
HGF - Programmthema: | Erforschung des Weltraums | ||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||
DLR - Forschungsgebiet: | R EW - Erforschung des Weltraums | ||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Exploration des Sonnensystems | ||||||||||||||||
Standort: | Berlin-Adlershof | ||||||||||||||||
Institute & Einrichtungen: | Institut für Planetenforschung > Experimentelle Planetenphysik | ||||||||||||||||
Hinterlegt von: | de Vera, Dr. Jean Pierre Paul | ||||||||||||||||
Hinterlegt am: | 06 Jan 2015 14:44 | ||||||||||||||||
Letzte Änderung: | 24 Apr 2024 20:00 |
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