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A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment

Müller, Berit and Koch, Dietmar and Lutz, Rainer and Schlegel, Karl and Treccani, Laura and Rezwan, Kurosch (2014) A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment. Materials Science and Engineering C: Materials for Biological Applications, 42, pp. 137-145. Elsevier. DOI: 10.1016/j.msec.2014.05.017 ISSN 0928-4931

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Official URL: http://dx.doi.org/10.1016/j.msec.2014.05.017

Abstract

Herein, we present a novel, freeze gelation-based one-pot process to directly incorporate active biorelevant compounds, such as proteins, during scaffold processing. The presented method is suitable to fabricate biocompatible, near-net, complex-shaped scaffolds with tunable porosity, degradability, and handling stability. In particular, the direct protein incorporation enabled a simultaneous adjustment and control of scaffold microstructure, porosity and enhancement of initial mechanical stability and resorbability. Two model proteins, serum albumin and lysozyme, are selected and their effect on scaffold stability and microstructure investigated by biaxial strength tests, electron microscopy, and mercury intrusion porosimetry. The resulting hydroxyapatite/protein composites feature adjustable porosities from 50 % to 70 % and a mechanical strength ranging from 2 to 6 MPa comparable to that of human spongiosa without sintering. Scaffold degradation behaviour and protein release are assessed by in vitro studies. A preliminary in vivo assessment of scaffold biocompatibility and resorption behaviour in adult domestic pigs is discussed. After implantation, composites are resorbed up to 50 % after only 4 weeks and up to 65 % after 8 weeks. In addition, 14 % new bone formation after 4 weeks and 37 % after 8 weeks are detected. All these investigations demonstrate the outstanding suitability of the one-pot-process to create, in a customisable and reliable way, biocompatible scaffolds with sufficient mechanical strength for handling and surgical insertion, and for potential use as biodegradable bone substitutes and versatile platform for local drug delivery.

Item URL in elib:https://elib.dlr.de/93489/
Document Type:Article
Title:A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Müller, BeritUniversität BremenUNSPECIFIED
Koch, Dietmardietmar.koch (at) dlr.deUNSPECIFIED
Lutz, RainerUniversität ErlangenUNSPECIFIED
Schlegel, KarlUniversität ErlangenUNSPECIFIED
Treccani, LauraUniversität BremenUNSPECIFIED
Rezwan, KuroschUniversität BremenUNSPECIFIED
Date:2014
Journal or Publication Title:Materials Science and Engineering C: Materials for Biological Applications
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:42
DOI :10.1016/j.msec.2014.05.017
Page Range:pp. 137-145
Publisher:Elsevier
ISSN:0928-4931
Status:Published
Keywords:biomimetic material, bioresorption, bone graft, hydroxyapatite composite, porosity, protein
HGF - Research field:other
HGF - Program:other
HGF - Program Themes:other
DLR - Research area:no assignment
DLR - Program:no assignment
DLR - Research theme (Project):no assignment
Location: Stuttgart
Institutes and Institutions:Institute of Structures and Design > Ceramic Composite Structures
Deposited By: Koch, Dietmar
Deposited On:22 Dec 2014 16:17
Last Modified:08 Mar 2018 18:37

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