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The Influence of Simulated Microgravity on Purinergic Signaling Is Different between Individual Culture and Endothelial and Smooth Muscle Cell Coculture

Zhang, Yu and Lau, Patrick and Pansky, Andreas and Kassack, Matthias and Hemmersbach, Ruth and Tobiasch, Edda (2014) The Influence of Simulated Microgravity on Purinergic Signaling Is Different between Individual Culture and Endothelial and Smooth Muscle Cell Coculture. BioMed Research International, Volume (2014), pp. 1-11. Hindawi Publishing Corporation. DOI: 10.1155/2014/413708 ISBN ISSN 2314-6141 ISSN 2314-6133

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Official URL: http://dx.doi.org/10.1155/2014/413708

Abstract

Exposure to microgravity conditions causes cardiovascular deconditioning in astronauts during spaceflight. Until now, no specific drugs are available for countermeasure, since the underlying mechanism is largely unknown. Endothelial cells (ECs) and smooth muscle cells (SMCs) play key roles in various vascular functions, many of which are regulated by purinergic 2 (P2) receptors. However, their function in ECs and SMCs under microgravity conditions is still unclear. In this study, primary ECs and SMCs were isolated from bovine aorta and verified with specific markers. We show for the first time that the P2 receptor expression pattern is altered in ECs and SMCs after 24 h exposure to simulated microgravity using a clinostat. However, conditioned medium compensates this change in specific P2 receptors, for example, P2X7. Notably, P2 receptors such as P2X7 might be the important players during the paracrine interaction. Additionally, ECs and SMCs secreted different cytokines under simulated microgravity, leading into a pathogenic proliferation and migration. In conclusion, our data indicate P2 receptors might be important players responding to gravity changes in ECs and SMCs. Since some artificial P2 receptor ligands are applied as drugs, it is reasonable to assume that they might be promising candidates against cardiovascular deconditioning in the future.

Item URL in elib:https://elib.dlr.de/90892/
Document Type:Article
Additional Information:HGF SpaceLife Programme
Title:The Influence of Simulated Microgravity on Purinergic Signaling Is Different between Individual Culture and Endothelial and Smooth Muscle Cell Coculture
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Zhang, YuDLR Institut für Luft- und Raumfahrtmedizin (SpaceLife)UNSPECIFIED
Lau, PatrickDLR Institut für Luft- und Raumfahrtmedizin, DLR, CologneUNSPECIFIED
Pansky, AndreasHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Kassack, MatthiasUniversität DüsseldorfUNSPECIFIED
Hemmersbach, RuthDLR Institut für Luft- und Raumfahrtmedizin, Biomedizinisches Wissenschafts-Unterstützungszentrum, KölnUNSPECIFIED
Tobiasch, EddaHochschule Bonn-Rhein-Sieg, RheinbachUNSPECIFIED
Date:2014
Journal or Publication Title:BioMed Research International
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:Volume
DOI :10.1155/2014/413708
Page Range:pp. 1-11
Publisher:Hindawi Publishing Corporation
ISSN:2314-6133
ISBN:ISSN 2314-6141
Status:Published
Keywords:purinergic receptors, endothelial cells, smooth muscle cells, microgravity, cell cocultures, P2X, P2Y, cardiovascular deconditioning
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:21 Oct 2014 09:56
Last Modified:08 Mar 2018 18:36

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