elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Establishment of a Blood Vessel Cell Culture Model, Testing its Applicability in Microgravity and Drug Research and Examination of the Underlying Signaling Pathways

Zhang, Yu (2014) Establishment of a Blood Vessel Cell Culture Model, Testing its Applicability in Microgravity and Drug Research and Examination of the Underlying Signaling Pathways. Dissertation, Heinrich-Heine-University of Düsseldorf.

Full text not available from this repository.

Abstract

During space missions astronauts suffer from cardiovascular deconditioning,when they are exposed to microgravity conditions. Until now, no specific drugs are available for effective countermeasures, since the underlying mechanism is not completely understood. Endothelial cells (ECs) and smooth muscle cells (SMCs) play crucial roles in a variety of cardiovascular functions, many of which are regulated via P2 receptors. However, their function in ECs and SMCs under microgravity condition is still unknown. In this study, ECs and SMCs were isolated from bovine aorta and differentiated from human mesenchymal stem cells (hMSCs), respectively. Subsequently, the cells were verified based on specific markers. An altered P2 receptor expression pattern was detected during the commitment of hMSC towards ECs and SMCs. The administration of natural and artificial P2 receptor agonists and antagonists directly affected the differentiation process. By using EC growth medium as conditioned medium, a vessel cell model was created to culture SMCs and vice versa. Within this study, we were able to show for the first time that the expression of some P2 receptors were altered in ECs and SMCs grown for 24h under simulated microgravity conditions. On the other hand, in someP2 receptor expressions such as P2X7 conditioned medium compensated this change. In conclusion, our data show that P2 receptors play an important functional role in hMSC differentiation towards ECs and SMCs. Since some P2 receptor artificial ligands are already used as drugs for patients with cardiovascular diseases, it is reasonable to assume that in the future they might be promising candidates for treating cardiovascular deconditioning.

Item URL in elib:https://elib.dlr.de/90612/
Document Type:Thesis (Dissertation)
Title:Establishment of a Blood Vessel Cell Culture Model, Testing its Applicability in Microgravity and Drug Research and Examination of the Underlying Signaling Pathways
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Zhang, YuDLR-Institut für Luft- und Raumfahrtmedizin (SpaceLife)UNSPECIFIED
Date:June 2014
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Number of Pages:151
Status:Published
Keywords:Endothelial cells, smooth muscle cells, microgravity simulation, purinergic receptors
Institution:Heinrich-Heine-University of Düsseldorf
Department:Faculty of Mathematics and Natural Science
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:16 Sep 2014 09:36
Last Modified:16 Sep 2014 09:36

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.