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Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1

Warnke, Elisabeth and Pietsch, Jessica and Wehland, Markus and Bauer, Johann and Infanger, Manfred and Görög, Mark and Hemmersbach, Ruth and Braun, Markus and Ma, Xiao and Sahana, Jayashree and Grimm, Daniela (2014) Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1. Cell Communication and Signaling, 12 (32), pp. 1-11. BioMed Central. ISSN 1478-811X

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Official URL: http://www.biosignaling.com/content/12/1/32

Abstract

Background: Multicellular tumor spheroids (MCTS) formed scaffold-free under microgravity are of high interest for research and medicine. Their formation mechanism can be studied in space in real microgravity or on Earth using ground-based facilities (GBF), which simulate microgravity. On Earth, these experiments are more cost-efficient and easily performable. However, each GBF might exert device-specific and altered superimposingly gravity-dependent effects on the cells. Results: FTC-133 human thyroid cancer cells were cultivated on a 2D clinostat (CN) and a random positioning machine (RPM) and compared with corresponding 1 g control cells. Harvested cell samples were investigated by microscopy, quantitative realtime-PCR and Multi-Analyte Profiling. Spheroid formation and growth occurred during 72 h of cultivation on both devices. Cytokine secretion and gene activation patterns frequently altered in different ways, when the cells were cultured either on the RPM or the CN. A decreased expression of CAV1 and CTGF in MCTS compared to adherent cells was observed after cultivation on both machines. Conclusion: The development of MCTS proceeds similarly on the RPM and the CN resembling the situation observed under real microgravity conditions, while no MCTS formation was observed at 1 g under identical experimental conditions. Simultaneously, changes in the regulation of CTGF and CAV1 appeared in a comparable manner on both machines. A relationship between these molecules and MCTS formation is discussed.

Item URL in elib:https://elib.dlr.de/89517/
Document Type:Article
Title:Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Warnke, ElisabethClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Pietsch, JessicaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Görög, MarkGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Braun, MarkusGravitational Biology, IMBIO, University of Bonn, GermanyUNSPECIFIED
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Sahana, JayashreeInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, DenmarkUNSPECIFIED
Date:10 May 2014
Journal or Publication Title:Cell Communication and Signaling
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
Volume:12
Page Range:pp. 1-11
Editors:
EditorsEmail
Feller, StephanMartin-Luther-University Halle-Wittenberg, Germany
Publisher:BioMed Central
ISSN:1478-811X
Status:Published
Keywords:Adherent growth, Three-dimensional growth, Cytokine, Microgravity, MCTS, RPM, 2D clinostat
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Bonn-Oberkassel
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Anken, Ralf
Deposited On:24 Jun 2014 10:23
Last Modified:21 Sep 2019 05:06

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