elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Imaging of nuclear factor κB activation induced by ionizing radiation in human embryonic kidney (HEK) cells

Chishti, Arif Ali and Baumstark-Khan, Christa and Hellweg, Christine E. and Reitz, Günther (2014) Imaging of nuclear factor κB activation induced by ionizing radiation in human embryonic kidney (HEK) cells. Radiation and Environmental Biophysics, 53, pp. 599-610. Springer. DOI: 10.1007/s00411-014-0541-x ISSN 0301-634X

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1007/s00411-014-0541-x

Abstract

Ionizing radiation modulates several signaling pathways resulting in transcription factor activation. Nuclear factor kappa B (NF-κB) is one of the most important transcription factors that respond to changes in the environment of a mammalian cell. NF-κB plays a key role not only in inflammation and immune regulation but also in cellular radiation response. In response to DNA damage, NF-κB might inhibit apoptosis and promote carcinogenesis. Our previous studies showed that ionizing radiation is very effective in inducing biological damages. Therefore, it is important to understand the radiation-induced NF-κB signaling cascade. The current study aims to improve existing mammalian cell-based reporter assays for NF-κB activation by the use of DD-tdTomato which is a destabilized variant of red fluorescent protein tdTomato. It is demonstrated that exposure of recombinant human embryonic kidney cells (HEK/293 transfected with a reporter constructs containing NF-κB binding sites in its promoter) to ionizing radiation induces NF-κB-dependent DD-tdTomato expression. Using this reporter assays, NF-κB signaling in mammalian cells was monitored by flow cytometry and fluorescence microscopy. Activation of NF-κB by the canonical pathway was found to be quicker than by the genotoxin- and stress-induced pathway. X-rays activate NF-κB in HEK cells in a dose-dependent manner, and the extent of NF-κB activation is higher as compared to camptothecin.

Item URL in elib:https://elib.dlr.de/89404/
Document Type:Article
Title:Imaging of nuclear factor κB activation induced by ionizing radiation in human embryonic kidney (HEK) cells
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Chishti, Arif AliRadiation Biology, Institute of Aerospace Medicine, German Aerospace Centre (DLR), Linder Höhe, 51147 Köln, Germany; arif.chishti (at) dlr.deUNSPECIFIED
Baumstark-Khan, ChristaRadiation Biology, Institute of Aerospace Medicine, German Aerospace Centre (DLR), Linder Höhe, 51147 Köln, GermanyUNSPECIFIED
Hellweg, Christine E.Radiation Biology, Institute of Aerospace Medicine, German Aerospace Centre (DLR), Linder Höhe, 51147 Köln, Germany; christine.hellweg (at) dlr.deUNSPECIFIED
Reitz, GüntherRadiation Biology, Institute of Aerospace Medicine, German Aerospace Centre (DLR), Linder Höhe, 51147 Köln, GermanyUNSPECIFIED
Date:2014
Journal or Publication Title:Radiation and Environmental Biophysics
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:53
DOI :10.1007/s00411-014-0541-x
Page Range:pp. 599-610
Publisher:Springer
Series Name:(Published online 01 June 2014)
ISSN:0301-634X
Status:Published
Keywords:Nuclear factor κB, Reporter system, Fluorescent proteins, Ionizing radiation, X-rays
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jun 2014 09:32
Last Modified:06 Sep 2019 15:19

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.