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Protein patterns of black fungi under simulated Mars-like conditions

Zakharova, K. and Marzban, G. and de Vera, Jean Pierre Paul and Lorek, Andreas and Sterflinger, K. (2014) Protein patterns of black fungi under simulated Mars-like conditions. Scientific Reports, 4, p. 5114. Nature Publishing Group. DOI: 10.1038/srep05114 ISSN 2045-2322

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Official URL: http://www.nature.com/srep/2014/140529/srep05114/full/srep05114.html

Abstract

Two species of microcolonial fungi – Cryomyces antarcticus and Knufia perforans - and a species of black yeasts–Exophiala jeanselmei - were exposed to thermo-physical Mars-like conditions in the simulation chamber of the German Aerospace Center. In this study the alterations at the protein expression level from various fungi species under Mars-like conditions were analyzed for the first time using 2D gel electrophoresis. Despite of the expectations, the fungi did not express any additional proteins under Mars simulation that could be interpreted as stress induced HSPs. However, up-regulation of some proteins and significant decreasing of protein number were detected within the first 24 hours of the treatment. After 4 and 7 days of the experiment protein spot number was increased again and the protein patterns resemble the protein patterns of biomass from normal conditions. It indicates the recovery of the metabolic activity under Martian environmental conditions after one week of exposure.

Item URL in elib:https://elib.dlr.de/89319/
Document Type:Article
Title:Protein patterns of black fungi under simulated Mars-like conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Zakharova, K.University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, A - 1190 Vienna, AustriaUNSPECIFIED
Marzban, G.University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, A - 1190 Vienna, AustriaUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Lorek, AndreasAndreas.Lorek (at) dlr.dehttps://orcid.org/0000-0002-8960-6214
Sterflinger, K.University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, A - 1190 Vienna, AustriaUNSPECIFIED
Date:29 May 2014
Journal or Publication Title:Scientific Reports
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:4
DOI :10.1038/srep05114
Page Range:p. 5114
Publisher:Nature Publishing Group
ISSN:2045-2322
Status:Published
Keywords:2D-electrophoresis, black fungi, Mars simulation, Astrobiology, protein patterns
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Experimentelle Planetenphysik
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:18 Jun 2014 11:23
Last Modified:01 Dec 2018 19:50

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