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Alterations in protein expression pattern induced by different gravitational stimuli in roots of Arabidopsis Thaliana

Schüler, Oliver and Röhrig, Horst and Menzel, Diedrik and Hemmersbach, Ruth (2013) Alterations in protein expression pattern induced by different gravitational stimuli in roots of Arabidopsis Thaliana. ELGRA Biennial Symposium and General Assembly, 11.-14. Sep. 2013, Rom, Italien. (Unpublished)

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Abstract

Plants will be an essential part of future Life Support Systems, which enable long duration human spaceflight and colonisation of other planets. For this reason we have to understand plant’s gravitational response towards a deviation from the vertical (e.g 90° alteration) and modifications/ behavioural changes under microgravity. The concrete objective of our work was to identify proteins, putative key proteins of the graviresponse, whose expression patterns are altered in Arabidopisis thaliana roots of 7 days old Col-0 wildtype seedlings after 30 minutes of simulated microgravity and 90° treatments. Total protein extracts were used for a quantitative analysis basing on 2D SDS-PAGE (KAMADA ET AL. 2005). All identified up- and downregulated proteins can be categorized in stress, transcriptional and translational modification, calcium signalling, auxin transporting and carbohydrate cycle and processing groups. Interestingly, some of the identified proteins exhibited an increased expression to a lack of gravity and a proper gravitational response. Our data clearly demonstrate several alterations of the proteome after 30 minutes of simulated microgravity and less changes after 30 minutes 90° stimulus. With the help of those insights and future experiments, we are able to prepare plants by breeding or genetic modification to grow, germinate and reproduce themselves in space with similar effectivity to earth.

Item URL in elib:https://elib.dlr.de/87876/
Document Type:Conference or Workshop Item (Speech)
Title:Alterations in protein expression pattern induced by different gravitational stimuli in roots of Arabidopsis Thaliana
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Schüler, OliverGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, GermanyUNSPECIFIED
Röhrig, HorstUniversity of Bonn, IMBIOUNSPECIFIED
Menzel, DiedrikUniversity of Bonn, IZMBUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Date:2013
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Unpublished
Keywords:Arabidopsis thaliana, Microgravity, Key proteins, Proteomics
Event Title:ELGRA Biennial Symposium and General Assembly
Event Location:Rom, Italien
Event Type:international Conference
Event Dates:11.-14. Sep. 2013
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Bromeis, Birgit
Deposited On:04 Feb 2014 15:32
Last Modified:04 Feb 2014 15:32

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