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Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine

Aleshcheva, Ganna and Sahana, Jayashree and Ma, Xiao and Hauslage, Jens and Hemmersbach, Ruth and Egli, Marcel and Infanger, Manfred and Bauer, Johann and Grimm, Daniela (2013) Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine. PLoS One, 8 (11). Public Library of Science (PLoS). DOI: 10.1371/journal.pone.0079057 ISSN 1932-6203

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Abstract

Tissue engineering with chondrocytes on the random positioning machine (RPM) is a new strategy of cartilage regeneration. Using a three-dimensional RPM, a device designed to simulate microgravity on Earth, we investigated the early effects of RPM exposure on human chondrocytes of six different donors after 30 minutes, 2 h, 4 h, 16 h, 24 h and compared the achieved results with the corresponding static controls cultured under normal gravity conditions. Already 30 minutes of RPM exposure resulted in an increased expression of several genes for cell motility, structure and integrity (beta-actin), for control of cell growth, cell proliferation, cell differentiation and apoptosis (TGF-β1, osteopontin) and altered cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours of RPM exposure even disruptions in the vimentin network could be detected. These changes were less dramatic after 16 hours on the RPM when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-β 1 was clearly enhanced during RPM culture for 24 h. Taken these results together we suggest that chondrocytes exposed to the RPM seem to change their extracellular matrix production behavior, while they rearrange their cytoskeletal proteins prior to forming 3D aggregates.

Item URL in elib:https://elib.dlr.de/87842/
Document Type:Article
Title:Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Aleshcheva, GannaSpacelife, Otto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Sahana, JayashreeInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Egli, MarcelAerospace Biomedical Science & Technology, Space Biology Group, Lucerne School of Engineering and Architecture, CH 6052 Hergiswil, SwitzerlandUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, GermanyUNSPECIFIED
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, DenmarkUNSPECIFIED
Date:11 November 2013
Journal or Publication Title:PLoS One
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:8
DOI :10.1371/journal.pone.0079057
Editors:
EditorsEmail
Agarwal, SudhaOhio State University, United States of America
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Status:Published
Keywords:chondrocytes, cartilage, cytoskeleton, extracellular matrix, microgravity
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Bromeis, Birgit
Deposited On:07 Feb 2014 13:16
Last Modified:08 Mar 2018 18:49

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