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The oxidative burst reaction in mammalian cells depends on gravity

Adrian, Astrid and Schoppmann, Kathrin and Sromicki, Juri and Brungs, Sonja and von der Wiesche, Melanie and Hock, Bertold and Kolanus, Waldemar and Hemmersbach, Ruth and Ullrich, Oliver (2013) The oxidative burst reaction in mammalian cells depends on gravity. Cell Communication and Signaling, 98 (11), pp. 1-20. BioMed Central. DOI: 10.1186/1478-811X-11-98 ISSN 1478-811X

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Official URL: http://www.biosignaling.com/content/11/1/98

Abstract

Gravity has been a constant force throughout the Earth’s evolutionary history. Thus, one of the fundamental biological questions is if and how complex cellular and molecular functions of life on Earth require gravity. In this study, we investigated the influence of gravity on the oxidative burst reaction in macrophages, one of the key elements in innate immune response and cellular signaling. An important step is the production of superoxide by the NADPH oxidase, which is rapidly converted to H2O2 by spontaneous and enzymatic dismutation. The phagozytosis-mediated oxidative burst under altered gravity conditions was studied in NR8383 rat alveolar macrophages by means of a luminol assay. Ground-based experiments in “functional weightlessness” were performed using a 2 D clinostat combined with a photomultiplier (PMT clinostat). The same technical set-up was used during the 13th DLR and 51st ESA parabolic flight campaign. Furthermore, hypergravity conditions were provided by using the Multi-Sample Incubation Centrifuge (MuSIC) and the Short Arm Human Centrifuge (SAHC). The results demonstrate that release of reactive oxygen species (ROS) during the oxidative burst reaction depends greatly on gravity conditions. ROS release is 1.) reduced in microgravity, 2.) enhanced in hypergravity and 3.) responds rapidly and reversible to altered gravity within seconds. We substantiated the effect of altered gravity on oxidative burst reaction in two independent experimental systems, parabolic flights and 2D clinostat / centrifuge experiments. Furthermore,the results obtained in simulated microgravity (2D clinorotation experiments) were proven by experiments in real microgravity as in both cases a pronounced reduction in ROS was observed. Our experiments indicate that gravity-sensitive steps are located both in the initial activation pathways and in the final oxidative burst reaction itself, which could be explained by the role of cytoskeletal dynamics in the assembly and function of the NADPH oxidase complex.

Item URL in elib:https://elib.dlr.de/87838/
Document Type:Article
Title:The oxidative burst reaction in mammalian cells depends on gravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Adrian, AstridDepartment of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University MagdeburgUNSPECIFIED
Schoppmann, KathrinLudwig-Maximilians University München, Dep. of Biology II, München, GermanyUNSPECIFIED
Sromicki, JuriUniversity of Zurich, Institute of AnatomyUNSPECIFIED
Brungs, SonjaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
von der Wiesche, MelanieGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hock, BertoldTechnische Universität München, Lehrstuhl für BioanalytikUNSPECIFIED
Kolanus, WaldemarLife and Medical Sciences (LIMES) Institute, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115 Bonn, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Ullrich, OliverUniversity of Zurich, Institute of Anatomy, Zurich, SwitzerlandUNSPECIFIED
Date:20 December 2013
Journal or Publication Title:Cell Communication and Signaling
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
Volume:98
DOI :10.1186/1478-811X-11-98
Page Range:pp. 1-20
Publisher:BioMed Central
Series Name:Biosignaling
ISSN:1478-811X
Status:Published
Keywords:Phagocytosis, Macrophage, Parabolic flight, Clinorotation, Hypergravity, Microgravity
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:16 Jun 2014 11:59
Last Modified:21 Sep 2019 05:06

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