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Insights into the Microbial Diversity and Bioburden in a South American Spacecraft Assembly Clean Room

Schwendner, Petra and Moissl-Eichinger, Christine and Barczyk, Simon and Bohmeier, Maria and Pukall, Rüdiger and Rettberg, Petra (2013) Insights into the Microbial Diversity and Bioburden in a South American Spacecraft Assembly Clean Room. Astrobiology, 13 (12), pp. 1140-1154. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2013.1023. ISSN 1531-1074

Full text not available from this repository.

Abstract

In this study, samples from the spacecraft assembly clean room BAF (final assembly building), located at Centre Spatial Guyanais in Kourou, French Guiana, were characterized by qualitative and quantitative methods to determine the bioburden and biodiversity. The cultivation assays mainly focused on extremotolerant microorganisms that have special metabolic skills, such as the ability to grow without oxygen, fix nitrogen, grow autotrophically, or reduce sulfate. A broad range of media and growth conditions were used to simulate possible extraterrestrial environments and clean room buildings. In addition to these alternative cultivation assays, the ESA standard protocol for bioburden estimation was also applied. The phylogenetic analysis of the isolates (mainly facultative anaerobes) showed an extraordinarily broad cultivable biodiversity. Overall, 49 species were isolated and identified as members of the bacterial phyla Actinobacteria, Firmicutes, α-, β-, γ-Proteobacteria, and Bacteroidetes/Chlorobi. In addition to cultivation-based analyses, molecular techniques were also applied, including construction of a 16S rRNA gene clone library. The results indicate a wide-ranging microbial diversity (12 bacterial phyla, 34 families) that not only confirms the results of the cultivation efforts but also deepens our understanding of the noncultivable variety. Our investigations hint at a very broad, mainly uncultivated microbial diversity.

Item URL in elib:https://elib.dlr.de/86905/
Document Type:Article
Title:Insights into the Microbial Diversity and Bioburden in a South American Spacecraft Assembly Clean Room
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Schwendner, PetraInstitute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany and German Aerospace Center (DLR), Köln, Germany.UNSPECIFIED
Moissl-Eichinger, ChristineInstitute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.UNSPECIFIED
Barczyk, SimonGerman Aerospace Center (DLR), Köln, Germany.UNSPECIFIED
Bohmeier, MariaGerman Aerospace Center (DLR), Köln, Germany.UNSPECIFIED
Pukall, RüdigerLeibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.UNSPECIFIED
Rettberg, PetraGerman Aerospace Center (DLR), Köln, Germany.UNSPECIFIED
Date:2013
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:13
DOI :10.1089/ast.2013.1023.
Page Range:pp. 1140-1154
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Planetary protection, Spacecraft assembly clean rooms, Microbial diversity, Anaerobes, FISH
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:18 Dec 2013 11:36
Last Modified:06 Sep 2019 15:23

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