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Resistance of Bacillus subtilis spore DNA to lethal ionizing radiation damage relies primarily on spore core components and DNA repair, with minor effects of oxygen radical detoxification

Moeller, Ralf and Raguse, Marina and Reitz, Günther and Okayasu, Ryuichi and Li, Zuofeng and Klein, Stuart and Setlow, Peter and Nicholson, Wayne L. (2013) Resistance of Bacillus subtilis spore DNA to lethal ionizing radiation damage relies primarily on spore core components and DNA repair, with minor effects of oxygen radical detoxification. Applied and Environmental Microbiology, 80 (1), pp. 104-109. American Society for Microbiology. DOI: 10.1128/AEM.03136-13 ISSN 0099-2240

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Abstract

The roles of various core components (α/β/γ-type small acid-soluble spore proteins (SASP), dipicolinic acid (DPA), core water content) and DNA repair by apurinic/apyrimidinic (AP) endonucleases or non-homologous end joining (NHEJ) in Bacillus subtilis spore resistance to different types of ionizing radiation including X-rays, protons, and high energy charged iron ions have been studied. Spores deficient in DNA repair by NHEJ or AP endonucleases, the oxidative stress response, or protection by major α/β-type SASP, DPA and decreased core water content were significantly more sensitive to ionizing radiation than wild-type spores, with highest sensitivity to high energy charged iron ions. DNA repair via NHEJ and AP endonucleases appears to be the most important mechanism for spore resistance to ionizing radiation, whereas oxygen radical detoxification via the MrgA-mediated oxidative stress response or KatX catalase activity plays only a very minor role. Synergistic radio-protective effects of α/β-type but not γ-type SASP were also identified, indicating that α/β-type SASP's binding to spore DNA is important in preventing DNA damage due to reactive oxygen species generated by ionizing radiation.

Item URL in elib:https://elib.dlr.de/86887/
Document Type:Article
Title:Resistance of Bacillus subtilis spore DNA to lethal ionizing radiation damage relies primarily on spore core components and DNA repair, with minor effects of oxygen radical detoxification
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), GermanyUNSPECIFIED
Raguse, MarinaGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), GermanyUNSPECIFIED
Reitz, GüntherGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), GermanyUNSPECIFIED
Okayasu, RyuichiInternational Open Laboratory, National Institute of Radiological Sciences, Chiba-shi, JapanUNSPECIFIED
Li, ZuofengUniversity of Florida, Proton Therapy Institute, Jacksonville, Florida, USAUNSPECIFIED
Klein, StuartUniversity of Florida, Proton Therapy Institute, Jacksonville, Florida, USAUNSPECIFIED
Setlow, PeterDepartment of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USAUNSPECIFIED
Nicholson, Wayne L.Department of Microbiology and Cell Science, University of Florida, Merritt Island, Florida, USAUNSPECIFIED
Date:2013
Journal or Publication Title:Applied and Environmental Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:80
DOI :10.1128/AEM.03136-13
Page Range:pp. 104-109
Publisher:American Society for Microbiology
ISSN:0099-2240
Status:Published
Keywords:Bacillus subtilis spore, ionizing radiation, resistance, DNA repair
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:18 Dec 2013 11:35
Last Modified:08 Jan 2014 13:31

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