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Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break

Vlašić, Ignacija and Mertens, Ramona and Seco, Elena M. and Carrasco, Begoña and Ayora, Silvia and Reitz, Günther and Commichau, Fabian M. and Alonso, Juan C. and Moeller, Ralf (2013) Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break. Nucleic Acids Research, Epub a. Oxford University Press. doi: 10.1093/nar/gkt1194. ISSN 0305-1048.

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Abstract

Bacillus subtilis RecA is important for spore resistance to DNA damage, even though spores contain a single non-replicating genome. We report that inactivation of RecA or its accessory factors, RecF, RecO, RecR and RecX, drastically reduce survival of mature dormant spores to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs). The presence of non-cleavable LexA renders spores less sensitive to DSBs, and spores impaired in DSB recognition or end-processing show sensitivities to X-rays similar to wild-type. In vitro RecA cannot compete with SsbA for nucleation onto ssDNA in the presence of ATP. RecO is sufficient, at least in vitro, to overcome SsbA inhibition and stimulate RecA polymerization on SsbA-coated ssDNA. In the presence of SsbA, RecA slightly affects DNA replication in vitro, but addition of RecO facilitates RecA-mediated inhibition of DNA synthesis. We propose that repairing of the DNA lesions generates a replication stress to germinating spores, and the RecA·ssDNA filament might act by preventing potentially dangerous forms of DNA repair occurring during replication. RecA might stabilize a stalled fork or prevent or promote dissolution of reversed forks rather than its cleavage that should require end-processing.

Item URL in elib:https://elib.dlr.de/86198/
Document Type:Article
Title:Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Vlašić, IgnacijaRadiation Biology Department, German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, D-51147 Cologne (Köln), Germany and Division of Molecular Biology, Laboratory of Evolutionary Genetics, Ruder Boskovic Institute, Zagreb, CroatiaUNSPECIFIED
Mertens, RamonaRadiation Biology Department, German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, D-51147 Cologne (Köln), GermanyUNSPECIFIED
Seco, Elena M.Department of Microbial Biotechnology, Centro Nacional de Biotecnologia, CSIC, Darwin 3, 28049 Madrid, SpainUNSPECIFIED
Carrasco, BegoñaDepartment of Microbial Biotechnology, Centro Nacional de Biotecnologia, CSIC, Darwin 3, 28049 Madrid, SpainUNSPECIFIED
Ayora, SilviaDepartment of Microbial Biotechnology, Centro Nacional de Biotecnologia, CSIC, Darwin 3, 28049 Madrid, SpainUNSPECIFIED
Reitz, GüntherRadiation Biology Department, German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, D-51147 Cologne (Köln), GermanyUNSPECIFIED
Commichau, Fabian M.Department of General Microbiology, University of Göttingen, Grisebachstrasse 8, D-37077 Göttingen, GermanyUNSPECIFIED
Alonso, Juan C.Department of Microbial Biotechnology, Centro Nacional de Biotecnologia, CSIC, Darwin 3, 28049 Madrid, SpainUNSPECIFIED
Moeller, RalfRadiation Biology Department, German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, D-51147 Cologne (Köln), GermanyUNSPECIFIED
Date:2013
Journal or Publication Title:Nucleic Acids Research
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:Epub a
DOI :10.1093/nar/gkt1194
Publisher:Oxford University Press
ISSN:0305-1048
Status:Published
Keywords:Bacillus subtilis, spore resistance, DNA damage, ultrahigh vacuum desiccation, ionizing radiation, RecA
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:18 Dec 2013 11:34
Last Modified:18 Dec 2013 11:34

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