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Cell cycle delay in murine pre-osteoblasts is more pronounced after exposure to high-LET compared to low-LET radiation

Hu, Yueyuan and Hellweg, Christine E. and Baumstark-Khan, Christa and Reitz, Günther and Lau, Patrick (2013) Cell cycle delay in murine pre-osteoblasts is more pronounced after exposure to high-LET compared to low-LET radiation. Radiation and Environmental Biophysics, 53 (1), pp. 73-81. Springer. DOI: 10.1007/s00411-013-0499-0 ISSN 0301-634X

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Abstract

Space radiation contains a complex mixture of particles comprised primarily of protons and high-energy heavy ions. Radiation risk is considered one of the major health risks for astronauts who embark on both orbital and interplanetary space missions. Ionizing radiation dosedependently kills cells, damages genetic material, and disturbs cell differentiation and function. The immediate response to ionizing radiation-induced DNA damage is stimulation of DNA repair machinery and activation of cell cycle regulatory checkpoints. To date, little is known about cell cycle regulation after exposure to space-relevant radiation, especially regarding bone-forming osteoblasts. Here, we assessed cell cycle regulation in the osteoblastic cell line OCT-1 after exposure to various types of space-relevant radiation. The relative biological effectiveness (RBE) of ionizing radiation was investigated regarding the biological endpoint of cellular survival ability. Cell cycle progression was examined following radiation exposure resulting in different RBE values calculated for a cellular survival level of 1 %. Our findings indicate that radiation with a linear energy transfer (LET) of 150 keV/μm was most effective in inducing reproductive cell killing by causing cell cycle arrest. Expression analyses indicated that cells exposed to ionizing radiation exhibited significantly up-regulated p21(CDKN1A) gene expression. In conclusion, our findings suggest that cell cycle regulation is more sensitive to high- LET radiation than cell survival, which is not solely regulated through elevated CDKN1A expression.

Item URL in elib:https://elib.dlr.de/85527/
Document Type:Article
Title:Cell cycle delay in murine pre-osteoblasts is more pronounced after exposure to high-LET compared to low-LET radiation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hu, YueyuanDivision of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Hellweg, Christine E.Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Baumstark-Khan, ChristaDivision of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Reitz, GüntherDivision of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Lau, PatrickDivision of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Date:2013
Journal or Publication Title:Radiation and Environmental Biophysics
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:53
DOI :10.1007/s00411-013-0499-0
Page Range:pp. 73-81
Publisher:Springer
Series Name:Epub 2013 - Print 2014
ISSN:0301-634X
Status:Published
Keywords:Ionizing radiation, RBE, Osteoblastic cells, DNA damage, Cell cycle, Transcription factors, Gene expression
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:21 Nov 2013 15:10
Last Modified:06 Sep 2019 15:19

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