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DNA-damage, survival, differentiation, and matrix mineralization in vitro of a murine multipotent mesenchymal precursor cell line

Lau, Patrick and Hu, Yueyuan and Hellweg, Christine and Baumstark-Khan , Christa and Reitz, Guenther (2013) DNA-damage, survival, differentiation, and matrix mineralization in vitro of a murine multipotent mesenchymal precursor cell line. In: Bone Abstracts (ISSN 2052-1219 (online)), 1 (PP185). ©2013 Bioscientifica. European Calcified Tissue Society Congress 2013, 18.-21. May 2013, Lisbon, Portugal. ISSN 2052-1219 (online)

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Abstract

Radiation therapy is one of the most effective and indispensable treatment modalities for cancer patients. Known tissue complications caused by radiation-induced stem cell depletion, may result in structural and functional alterations of the surrounding matrix. Although, studies have demonstrated that ionizing radiation can induce apoptosis and senescence, little is known about the effects of therapeutic irradiation concerning the commitment of mesenchymal stem cells to the osteoblastic lineage. C3H10T1/2 clone eight cells were used reflecting an early stage of differentiation. Notably, radiation doses of 2 Gy reduced proliferation, but had no significant effect on cell viability. Cell cycle analysis revealed that the yield of cells captured in the G₂/M phase of the cell cycle was markedly and dose-dependently increased. Instead of apoptosis we detected increased activity of stress-induced premature cellular senescence. Histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix revealed positive staining for alizarin red S. Expression of TP53 encoding for tumour suppressor protein p53 and its downstream target cyclin-dependent kinase inhibitor 1A (p21(Cip1/Waf1)) were significantly increased. Gene expression analysis of two osteoblast specific genes, Runx2 and osteocalcin were assessed. Here, we confirmed that exposure to X-rays was dose dependently effective in decreasing cellular survival. Our results indicate that the direct impairment of proliferation and osteogenic differentiation potential of MSCs by irradiation may contribute partly to post-irradiation osteoporosis.

Item URL in elib:https://elib.dlr.de/85302/
Document Type:Conference or Workshop Item (Poster)
Title:DNA-damage, survival, differentiation, and matrix mineralization in vitro of a murine multipotent mesenchymal precursor cell line
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Lau, Patrick German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Hu, YueyuanGerman Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Hellweg, Christine German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Baumstark-Khan , Christa German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Reitz, Guenther German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Date:2013
Journal or Publication Title:Bone Abstracts (ISSN 2052-1219 (online))
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:1
Editors:
EditorsEmail
Langdahl, Bente (Chair)ECTS 2013 Scientific Programme Committee
Publisher:©2013 Bioscientifica
ISSN:2052-1219 (online)
Status:Published
Keywords:DNA-damage, ionizing radiation, radiation therapy, osteoblast specific genes, mesenchymal precursor cell line
Event Title:European Calcified Tissue Society Congress 2013
Event Location:Lisbon, Portugal
Event Type:international Conference
Event Dates:18.-21. May 2013
Organizer:European Calcified Tissue Society
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:15 Nov 2013 12:24
Last Modified:08 May 2014 23:25

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