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Involvement of Runx2 in the differentiation process of osteoblastic precursor cells after radiation exposure

Hu, Yueyuan and Lau, Patrick and Hellweg, Christine and Baumstark-Khan , Christa and Reitz, Guenther (2013) Involvement of Runx2 in the differentiation process of osteoblastic precursor cells after radiation exposure. In: Bone Abstracts (ISSN 2052-1219 (online)), 1 (PP186 ). ©2013 Bioscientifica. European Calcified Tissue Society Congress 2013, 18.-21. May 2013, Lisbon, Portugal. ISSN 2052-1219 (online)

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Abstract

Astronauts on exploratory space missions will experience a complex environment that includes microgravity and radiation. While the deleterious effects of unloading on bone are well established, fewer studies have focused on the effects of radiation. Space radiation produces distinct biological damages which, up to now, little is known about the correlation between radiation exposure and bone tissue. In our study we used osteoblastic precursor cells to investigate the radiation response of bone cells. Effects of radiation on differentiation were investigated by their ability to deposit extracellular matrices that mineralize under in vitro culture conditions using the histochemical Alizarin Red Staining (ARS). Calcium precipitation was detected in a bright red color already ten days after exposure to X-rays for doses up to 10 Gy. Notably, our results indicate that exposure to higher radiation doses could be correlated to a pronounced staining of the extracellular matrix. In order to gain more detailed insights into the osteoblast specific mineralization process, the transcriptional expression level of Runx2 was analysed. Our studies suggest that space relevant radiation significantly modulates the mineralization process and effectively modulates the gene expression levels of Runx2 involved in the differentiation of osteoblasts. In conclusion, the presented data allow the suggestion that exposure to ionizing radiation interferes with bone formation at the level of cellular differentiation.

Item URL in elib:https://elib.dlr.de/85292/
Document Type:Conference or Workshop Item (Poster)
Title:Involvement of Runx2 in the differentiation process of osteoblastic precursor cells after radiation exposure
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hu, YueyuanGerman Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Lau, Patrick German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Hellweg, Christine German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Baumstark-Khan , Christa German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Reitz, Guenther German Aerospace Center (DLR), Institut of Aerospace Medicine, Cologne, Germany.UNSPECIFIED
Date:2013
Journal or Publication Title:Bone Abstracts (ISSN 2052-1219 (online))
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:1
Editors:
EditorsEmail
Langdahl, Bente (Chair)ECTS 2013 Scientific Programme Committee
Publisher:©2013 Bioscientifica
ISSN:2052-1219 (online)
Status:Published
Keywords:Space radiation, osteoblastic precursor cells, Runx2, X-rays
Event Title:European Calcified Tissue Society Congress 2013
Event Location:Lisbon, Portugal
Event Type:international Conference
Event Dates:18.-21. May 2013
Organizer:European Calcified Tissue Society
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:15 Nov 2013 12:26
Last Modified:08 May 2014 23:25

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