elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Costamere remodeling with muscle loading and unloading in healthy young men

Li, Ruowei and Narici, Marco and Erskine, Robert and Seynnes, Olivier and Rittweger, Jörn and Pisot, Rado and Simunic, Bostjan and Flück, Martin (2013) Costamere remodeling with muscle loading and unloading in healthy young men. Journal of Anatomy (223), pp. 525-536. Wiley. DOI: doi: 10.1111/joa.12101 ISSN 0021-8782

[img]
Preview
PDF
1MB

Abstract

Costameres are mechano-sensory sites of focal adhesion in the sarcolemma that provide a structural anchor for myofibrils. Their turnover is regulated by integrin-associated focal adhesion kinase (FAK). We hypothesized that changes in content of costamere components (beta 1 integrin, FAK, meta-vinculin, gamma-vinculin) with increased and reduced loading of human anti-gravity muscle would: (i) relate to changes in muscle size and molecular parameters of muscle size regulation [p70S6K, myosin heavy chain (MHC)1 and MHCIIA]; (ii) correspond to adjustments in activity and expression of FAK, and its negative regulator, FRNK; and (iii) reflect the temporal response to reduced and increased loading. Unloading induced a progressive decline in thickness of human vastus lateralis muscle after 8 and 34 days of bedrest (-4% and -14%, respectively; n = 9), contrasting the increase in muscle thickness after 10 and 27 days of resistance training (+5% and +13%; n = 6). Changes in muscle thickness were correlated with changes in cross-sectional area of type I muscle fibers (r = 0.66) and beta 1 integrin content (r = 0.76) at the mid-point of altered loading. Changes in meta-vinculin and FAK-pY397 content were correlated (r = 0.85) and differed, together with the changes of beta 1 integrin, MHCI, MHCII and p70S6K, between the mid- and end-point of resistance training. By contrast, costamere protein level changes did not differ between time points of bedrest. The findings emphasize the role of FAKregulated costamere turnover in the load-dependent addition and removal of myofibrils, and argue for two phases of muscle remodeling with resistance training, which do not manifest at the macroscopic level.

Item URL in elib:https://elib.dlr.de/84628/
Document Type:Article
Title:Costamere remodeling with muscle loading and unloading in healthy young men
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Li, RuoweiUniversity, ManchesterUNSPECIFIED
Narici, MarcoUniversity ManchesterUNSPECIFIED
Erskine, RobertUniversity, ManchesterUNSPECIFIED
Seynnes, OlivierManchester Metropoliaton UniversityUNSPECIFIED
Rittweger, Jörnjoern.rittweger (at) dlr.deUNSPECIFIED
Pisot, RadoUniversity of PrimorskaUNSPECIFIED
Simunic, BostjanUniversity of PrimorskaUNSPECIFIED
Flück, MartinUniversity, ManchesterUNSPECIFIED
Date:8 September 2013
Journal or Publication Title:Journal of Anatomy
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :doi: 10.1111/joa.12101
Page Range:pp. 525-536
Publisher:Wiley
ISSN:0021-8782
Status:Published
Keywords:atrophy; focal adhesion kinase; human; hypertrophy; load; muscle; myofibril; vinculin
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Systemphysiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Becker, Christine
Deposited On:15 Nov 2013 12:43
Last Modified:31 Jul 2019 19:42

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.