Li, Ruowei und Narici, Marco und Erskine, Robert und Seynnes, Olivier und Rittweger, Jörn und Pisot, Rado und Simunic, Bostjan und Flück, Martin (2013) Costamere remodeling with muscle loading and unloading in healthy young men. Journal of Anatomy (223), Seiten 525-536. Wiley. doi: 10.1111/joa.12101. ISSN 0021-8782.
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Kurzfassung
Costameres are mechano-sensory sites of focal adhesion in the sarcolemma that provide a structural anchor for myofibrils. Their turnover is regulated by integrin-associated focal adhesion kinase (FAK). We hypothesized that changes in content of costamere components (beta 1 integrin, FAK, meta-vinculin, gamma-vinculin) with increased and reduced loading of human anti-gravity muscle would: (i) relate to changes in muscle size and molecular parameters of muscle size regulation [p70S6K, myosin heavy chain (MHC)1 and MHCIIA]; (ii) correspond to adjustments in activity and expression of FAK, and its negative regulator, FRNK; and (iii) reflect the temporal response to reduced and increased loading. Unloading induced a progressive decline in thickness of human vastus lateralis muscle after 8 and 34 days of bedrest (-4% and -14%, respectively; n = 9), contrasting the increase in muscle thickness after 10 and 27 days of resistance training (+5% and +13%; n = 6). Changes in muscle thickness were correlated with changes in cross-sectional area of type I muscle fibers (r = 0.66) and beta 1 integrin content (r = 0.76) at the mid-point of altered loading. Changes in meta-vinculin and FAK-pY397 content were correlated (r = 0.85) and differed, together with the changes of beta 1 integrin, MHCI, MHCII and p70S6K, between the mid- and end-point of resistance training. By contrast, costamere protein level changes did not differ between time points of bedrest. The findings emphasize the role of FAKregulated costamere turnover in the load-dependent addition and removal of myofibrils, and argue for two phases of muscle remodeling with resistance training, which do not manifest at the macroscopic level.
elib-URL des Eintrags: | https://elib.dlr.de/84628/ | ||||||||||||||||||||||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||||||||||||||||||
Titel: | Costamere remodeling with muscle loading and unloading in healthy young men | ||||||||||||||||||||||||||||||||||||
Autoren: |
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Datum: | 8 September 2013 | ||||||||||||||||||||||||||||||||||||
Erschienen in: | Journal of Anatomy | ||||||||||||||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||||||||||||||
Open Access: | Ja | ||||||||||||||||||||||||||||||||||||
Gold Open Access: | Nein | ||||||||||||||||||||||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1111/joa.12101 | ||||||||||||||||||||||||||||||||||||
Seitenbereich: | Seiten 525-536 | ||||||||||||||||||||||||||||||||||||
Verlag: | Wiley | ||||||||||||||||||||||||||||||||||||
ISSN: | 0021-8782 | ||||||||||||||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||||||||||||||
Stichwörter: | atrophy; focal adhesion kinase; human; hypertrophy; load; muscle; myofibril; vinculin | ||||||||||||||||||||||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||||||||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||||||||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||||||||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Vorhaben Systemphysiologie (alt) | ||||||||||||||||||||||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Weltraumphysiologie | ||||||||||||||||||||||||||||||||||||
Hinterlegt von: | Becker, Christine | ||||||||||||||||||||||||||||||||||||
Hinterlegt am: | 15 Nov 2013 12:43 | ||||||||||||||||||||||||||||||||||||
Letzte Änderung: | 04 Jul 2023 14:45 |
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