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Single-cell analysis of the methanogenic archaeon Methanosarcina soligelidi from Siberian permafrost by means of confocal Raman microspectrocopy for astrobiological research

Serrano, S. and Wagner, D. and Böttger, Ute and de Vera, Jean Pierre Paul and Lasch, P. and Hermelink, A. (2013) Single-cell analysis of the methanogenic archaeon Methanosarcina soligelidi from Siberian permafrost by means of confocal Raman microspectrocopy for astrobiological research. Planetary and Space Science, 98, pp. 191-197. Elsevier. DOI: 10.1016/j.pss.2013.10.002 ISSN 0032-0633

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Abstract

Methanogenic archaea from Siberian permafrost are suitable model organisms that meet many of the preconditions for survival on the martian subsurface. These microorganisms have proven to be highly resistant when exposed to diverse stress factors such as desiccation, radiation and other thermo-physical martian conditions. In addition, methanogenic archaea produce methane as a metabolic byproduct, which was reported to be present in some regions of the Red Planet. The ExoMars mission will deploy a rover carrying a Raman spectrometer among the analytical instruments in order to search for signatures of life and to investigate the martian geochemistry. Raman spectroscopy is known as a powerful nondestructive optical technique for biosignature detection that requires only little sample preparation. In this study, we describe the use of confocal Raman microspectroscopy (CRM) as a rapid and sensitive technique for characterization of the methanogenic archaeon Methanosarcina soligelidi SMA-21 at the single cell level. These studies involved acquisition of Raman spectra from individual cells isolated from microbial cultures at different stages of growth. Spectral analyses indicated a high degree of heterogeneity between cells of individual cultures and also demonstrated the existence of growth-phase specific Raman patterns. For example, besides common Raman patterns of microbial cells, CRM additionally revealed the presence of lipid vesicles and CaCO3 particles in microbial preparations of M. soligelidi SMA-21, a finding that could be confirmed by electron microscopy. The results of this study suggest that heterogeneity and diversity of microorganisms have to be considered when using Raman-based technologies in future space exploration missions.

Item URL in elib:https://elib.dlr.de/83653/
Document Type:Article
Title:Single-cell analysis of the methanogenic archaeon Methanosarcina soligelidi from Siberian permafrost by means of confocal Raman microspectrocopy for astrobiological research
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Serrano, S.Alfred Wegener Institute for Polar and Marine Research. Telegrafenberg A45 14473 Potsdam, GermanyUNSPECIFIED
Wagner, D.Alfred Wegener Institute for Polar and Marine Research, Potsdam, GermanyUNSPECIFIED
Böttger, UteUte.Boettger (at) dlr.deUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Lasch, P.Robert Koch Institute, Centre of biological Safety 6; Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
Hermelink, A.Robert Koch Institute. Nordufer 20 13353 Berlin, GermanyUNSPECIFIED
Date:9 October 2013
Journal or Publication Title:Planetary and Space Science
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:98
DOI :10.1016/j.pss.2013.10.002
Page Range:pp. 191-197
Publisher:Elsevier
ISSN:0032-0633
Status:Published
Keywords:methanogenic archaea, Methanosarcina, confocal Raman microspectroscopy, single-cell analysis
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Experimentelle Planetenphysik
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:28 Nov 2013 11:01
Last Modified:01 Dec 2018 19:49

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