elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Steep increase in myonuclear domain size during infancy.

Delhaas, T and van der Meer, S.F. and Schaart, G and Degens, H. and Drost, MR (2013) Steep increase in myonuclear domain size during infancy. Anatomical Record, pp. 192-197. Wiley. DOI: doi: 10.1002/ar.22631 ISSN 1932-8486

[img]
Preview
PDF
7MB

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/ar.22631/abstract

Abstract

We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 μm(2) ·per annum (R(2) = 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase.

Item URL in elib:https://elib.dlr.de/82719/
Document Type:Article
Title:Steep increase in myonuclear domain size during infancy.
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Delhaas, TMaastricht University Medical CentreUNSPECIFIED
van der Meer, S.F.MEUNSPECIFIED
Schaart, GMaastricht University Medical CentreUNSPECIFIED
Degens, H.MEUNSPECIFIED
Drost, MRMaastricht University Medical CentreUNSPECIFIED
Date:February 2013
Journal or Publication Title:Anatomical Record
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :doi: 10.1002/ar.22631
Page Range:pp. 192-197
Editors:
EditorsEmail
Albertine, Kurt H.UNSPECIFIED
Publisher:Wiley
ISSN:1932-8486
Status:Published
Keywords:skeletal muscle; myonuclei; satellite cell; myonuclear domain; muscle fiber size; maturation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Systemphysiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Becker, Christine
Deposited On:20 Jun 2013 10:03
Last Modified:06 Sep 2019 15:30

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.