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Multifactorial Resistance of Bacillus subtilis Spores to High-Energy Proton Radiation: Role of Spore Structural Components and the Homologous Recombination and Non-Homologous End Joining DNA Repair Pathways

Moeller, Ralf and Reitz, Günther and Li, Zuofeng and Klein, Stuart and Nicholson, Wayne L. (2012) Multifactorial Resistance of Bacillus subtilis Spores to High-Energy Proton Radiation: Role of Spore Structural Components and the Homologous Recombination and Non-Homologous End Joining DNA Repair Pathways. Astrobiology, 12 (11), pp. 1069-1077. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2012.0890

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Abstract

The space environment contains high-energy charged particles (e.g., protons, neutrons, electrons, α-particles, heavy ions) emitted by the Sun and galactic sources or trapped in the radiation belts. Protons constitute the majority (87%) of high-energy charged particles. Spores of Bacillus species are one of the model systems used for astro- and radiobiological studies. In this study, spores of different Bacillus subtilis strains were used to study the effects of high energetic proton irradiation on spore survival. Spores of the wild-type B. subtilis strain [mutants deficient in the homologous recombination (HR) and non-homologous end joining (NHEJ) DNA repair pathways and mutants deficient in various spore structural components such as dipicolinic acid (DPA), α/β type small, acidsoluble spore protein (SASP) formation, spore coats, pigmentation, or spore core water content] were irradiated as air-dried multilayers on spacecraft-qualified aluminum coupons with 218 MeV protons [with a linear energy transfer (LET) of 0.4 keV/μm] to various final doses up to 2500 Gy. Spores deficient in NHEJ- and HR-mediated DNA repair were significantly more sensitive to proton radiation than wild-type spores, indicating that both HR and NHEJ DNA repair pathways are needed for spore survival. Spores lacking DPA, α/β-type SASP, or with increased core water content were also significantly more sensitive to proton radiation, whereas the resistance of spores lacking pigmentation or spore coats was essentially identical to that of the wild-type spores. Our results indicate that α/β-type SASP, core water content, and DPA play an important role in spore resistance to high-energy proton irradiation, suggesting their essential function as radioprotectants of the spore interior.

Item URL in elib:https://elib.dlr.de/78328/
Document Type:Article
Title:Multifactorial Resistance of Bacillus subtilis Spores to High-Energy Proton Radiation: Role of Spore Structural Components and the Homologous Recombination and Non-Homologous End Joining DNA Repair Pathways
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Moeller, Ralf German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Cologne, Germany.UNSPECIFIED
Reitz, Günther German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Cologne, Germany.UNSPECIFIED
Li, Zuofeng University of Florida, Proton Therapy Institute, Jacksonville, Florida, USA.UNSPECIFIED
Klein, Stuart University of Florida, Proton Therapy Institute, Jacksonville, Florida, USA.UNSPECIFIED
Nicholson, Wayne L. University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA.UNSPECIFIED
Date:2012
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:12
DOI :10.1089/ast.2012.0890
Page Range:pp. 1069-1077
Publisher:Mary Ann Liebert Inc.
Status:Published
Keywords:Bacillus, Spores, DNA repair, Protection, High-energy proton radiation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:12 Nov 2012 09:15
Last Modified:06 Sep 2019 15:23

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