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Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions

Billi, Daniela and Viaggiu, Emanuela and Cockell, Charles S. and Rabbow, Elke and Horneck, Gerda and Onofri, Silvano (2011) Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions. Astrobiology, 11 (1), pp. 65-73. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2009.0430

Full text not available from this repository.

Abstract

The cyanobacterium Chroococcidiopsis, overlain by 3mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting.

Item URL in elib:https://elib.dlr.de/70836/
Document Type:Article
Title:Damage Escape and Repair in Dried Chroococcidiopsis spp. from Hot and Cold Deserts Exposed to Simulated Space and Martian Conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Billi, Daniela Dipartimento di Biologia, Università di Roma ‘‘Tor Vergata,’’ Roma, Italy.UNSPECIFIED
Viaggiu, Emanuela Dipartimento di Biologia, Università di Roma ‘‘Tor Vergata,’’ Roma, Italy.UNSPECIFIED
Cockell, Charles S. Open University, Milton Keynes, United Kingdom.UNSPECIFIED
Rabbow, Elke Institute of Aerospace Medicine, DLR, Cologne, GermanyUNSPECIFIED
Horneck, Gerda Institute of Aerospace Medicine, DLR, Cologne, GermanyUNSPECIFIED
Onofri, Silvano Dipartimento di Ecologia e Sviluppo Economico Sostenibile, Università della Tuscia, Viterbo, Italy.UNSPECIFIED
Date:2011
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:11
DOI :10.1089/ast.2009.0430
Page Range:pp. 65-73
Publisher:Mary Ann Liebert Inc.
Status:Published
Keywords:Astrobiology, Endolithic cyanobacteria, Chroococcidiopsis, Ground-based experiments
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:09 Sep 2011 09:08
Last Modified:06 Sep 2019 15:23

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