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Impact of two DNA repair pathways, homologous recombination and non-homologous end joining, on bacterial spore inactivation under simulated martian environmental conditions

Moeller, Ralf and Schuerger, Andrew C. and Reitz, Günther and Nicholson, Wayne L. (2011) Impact of two DNA repair pathways, homologous recombination and non-homologous end joining, on bacterial spore inactivation under simulated martian environmental conditions. Icarus: International Journal of Solar System Studies, 215, pp. 204-210. Elsevier. doi: 10.1016/j.icarus.2011.06.035.

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Abstract

Spores of Bacillus subtilis were used as a model system to study the impact of the two major DNA doublestrand break (DSB) repair mechanisms [homologous recombination (HR) and non-homologous end-joining (NHEJ)] on the survivability of air-dried mono- and multilayers of bacterial spores under a simulated martian environment; i.e., an environment with low temperature (-10°C), pure CO₂ atmosphere (99.99% CO₂), 200–1100 nm UV–VIS–NIR radiation, and 0.69 kPa pressure. Spores in multilayers exhibited low inactivation rates compared to monolayers, mainly due to shadowing effects of overlying spores. Simulated martian UV irradiation reduced dramatically spore viability, whereas when shielded from martian UV radiation, spores deficient in NHEJ- and HR-mediated DNA repair were significantly more sensitive to simulated martian environmental conditions than were wild-type spores. In addition, NHEJ-deficient spores were consistently more sensitive than HR-deficient spores to simulated Mars environmental conditions, suggesting that DSBs were an important type of DNA damage. The results indicated that both HR and NHEJ provide an efficient set of DNA repair pathways ensuring spore survival after exposure to simulated martian environmental conditions.

Item URL in elib:https://elib.dlr.de/70835/
Document Type:Article
Title:Impact of two DNA repair pathways, homologous recombination and non-homologous end joining, on bacterial spore inactivation under simulated martian environmental conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Moeller, Ralf German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Research Group ’Astrobiology’, Cologne (Köln), GermanyUNSPECIFIED
Schuerger, Andrew C. University of Florida, Department of Plant Pathology, Building M6-1025, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USAUNSPECIFIED
Reitz, Günther German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), GermanyUNSPECIFIED
Nicholson, Wayne L. University of Florida, Department of Microbiology and Cell Science, Building M6-1025, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USAUNSPECIFIED
Date:2011
Journal or Publication Title:Icarus: International Journal of Solar System Studies
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:215
DOI :10.1016/j.icarus.2011.06.035
Page Range:pp. 204-210
Publisher:Elsevier
Status:Published
Keywords:Astrobiology, Exobiology, Mars, Climate
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:09 Sep 2011 09:09
Last Modified:26 Mar 2013 13:30

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