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Effect of radioprotective agents in sporulation medium on Bacillus subtilis spore resistance to hydrogen peroxide, wet heat and germicidal and environmentally relevant UV radiation

Moeller, R. and Wassmann, M. and Reitz, G. and Setlow, P. (2011) Effect of radioprotective agents in sporulation medium on Bacillus subtilis spore resistance to hydrogen peroxide, wet heat and germicidal and environmentally relevant UV radiation. Journal of Applied Microbiology, 110 (6), pp. 1485-1494. Wiley. DOI: 10.1111/j.1365-2672.2011.05004.x ISSN 1364-5072

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Abstract

Aims: To determine the effects of cysteine, cystine, proline and thioproline as sporulation medium supplements on Bacillus subtilis spore resistance to hydrogen peroxide (H₂O₂), wet heat, and germicidal 254 nm and simulated environmental UV radiation. Methods and Results: Bacillus subtilis spores were prepared in a chemically defined liquid medium, with and without supplementation of cysteine, cystine, proline or thioproline. Spores produced with thioproline, cysteine or cystine were more resistant to environmentally relevant UV radiation at 280–400 and 320–400 nm, while proline supplementation had no effect. Spores prepared with cysteine, cystine or thioproline were also more resistant to H₂O₂ but not to wet heat or 254-nm UV radiation. The increases in spore resistance attributed to the sporulation supplements were eliminated if spores were chemically decoated. Conclusions: Supplementation of sporulation medium with cysteine, cystine or thioproline increases spore resistance to solar UV radiation reaching the Earth’s surface and to H₂O₂. These effects were eliminated if the spores were decoated, indicating that alterations in coat proteins by different sporulation conditions can affect spore resistance to some agents. Significance and Impact of the Study: This study provides further evidence that the composition of the sporulation medium can have significant effects on B. subtilis spore resistance to UV radiation and H₂O₂. This knowledge provides further insight into factors influencing spore resistance and inactivation.

Item URL in elib:https://elib.dlr.de/69865/
Document Type:Article
Title:Effect of radioprotective agents in sporulation medium on Bacillus subtilis spore resistance to hydrogen peroxide, wet heat and germicidal and environmentally relevant UV radiation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Moeller, R. ralf.moeller (at) dlr.deUNSPECIFIED
Wassmann, M. UNSPECIFIEDUNSPECIFIED
Reitz, G. UNSPECIFIEDUNSPECIFIED
Setlow, P. Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT, USAUNSPECIFIED
Date:2011
Journal or Publication Title:Journal of Applied Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:110
DOI :10.1111/j.1365-2672.2011.05004.x
Page Range:pp. 1485-1494
Publisher:Wiley
ISSN:1364-5072
Status:Published
Keywords:Bacillus, bacterial spores, environmental, radiation, resistance
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:16 Jun 2011 10:51
Last Modified:06 Sep 2019 15:21

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