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Role of the Nfo and ExoA Apurinic/Apyrimidinic Endonucleases in Radiation Resistance and Radiation-Induced Mutagenesis of Bacillus subtilis Spores

Moeller, Ralf and Setlow, Peter and Pedraza-Reyes, Mario and Okayasu, Ryuichi and Reitz, Günther and Nicholson, Wayne L. (2011) Role of the Nfo and ExoA Apurinic/Apyrimidinic Endonucleases in Radiation Resistance and Radiation-Induced Mutagenesis of Bacillus subtilis Spores. Journal of Bacteriology, 193 (11), pp. 2875-2879. American Society for Microbiology. doi: 10.1128/JB.00134-11.

Full text not available from this repository.

Abstract

The roles of DNA repair by apurinic/apyrimidinic (AP) endonucleases alone, and together with DNA protection by α/β-type small acid-soluble spore proteins (SASP), in Bacillus subtilis spore resistance to different types of radiation have been studied. Spores lacking both AP endonucleases (Nfo and ExoA) and major SASP were significantly more sensitive to 254-nm UV-C, environmental UV (>280 nm), X-ray exposure, and highenergy charged (HZE)-particle bombardment and had elevated mutation frequencies compared to those of wild-type spores and spores lacking only one or both AP endonucleases or major SASP. These findings further implicate AP endonucleases and α/β-type SASP in repair and protection, respectively, of spore DNA against effects of UV and ionizing radiation.

Item URL in elib:https://elib.dlr.de/69846/
Document Type:Article
Title:Role of the Nfo and ExoA Apurinic/Apyrimidinic Endonucleases in Radiation Resistance and Radiation-Induced Mutagenesis of Bacillus subtilis Spores
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Moeller, Ralf German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyUNSPECIFIED
Setlow, Peter Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030UNSPECIFIED
Pedraza-Reyes, Mario University of Guanajuato, Department of Biology (DNEC), Division of Natural and Exact Sciences, Guanajuato, MexicoUNSPECIFIED
Okayasu, Ryuichi National Institute of Radiological Sciences (NIRS), Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, Chiba-shi, JapanUNSPECIFIED
Reitz, GüntherGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne, GermanyUNSPECIFIED
Nicholson, Wayne L. University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, FloridaUNSPECIFIED
Date:2011
Journal or Publication Title:Journal of Bacteriology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:193
DOI :10.1128/JB.00134-11
Page Range:pp. 2875-2879
Publisher:American Society for Microbiology
Status:Published
Keywords:Bacillus subtilis Spores, Radiation Resistance, Radiation-Induced Mutagenesis, DNA repair, apurinic/apyrimidinic (AP) endonucleases, α/β-type small acid-soluble spore proteins (SASP), DNA protection
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:16 Jun 2011 10:53
Last Modified:04 Apr 2013 16:29

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