elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach

Agostini, Francesco and Dalla Libera, Luciano and Rittweger, Jörn and Mazzucco, Sara and Jurdana, Mihaela and Mekjavic, Igor and Pisot, Rado and Gorza, Luisa and Narici, Marco and Biolo, Gianni (2010) Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach. The Journal of Physiology, 588 (24), pp. 5089-5104. Wiley. ISSN 0022-3751

This is the latest version of this item.

[img]
Preview
PDF
479kB

Abstract

Oxidative stress is often associated to inactivity-mediated skeletal muscle atrophy. Glutathione is one of the major antioxidant systems stimulated, both at muscular and systemic level, by activation of oxidative processes. We measured changes in glutathione availability, oxidative stress induction and the extent of atrophy mediated by 35 days of experimental bed rest in vastus lateralis muscle of healthy human volunteers. To assess muscle glutathione synthesis, we applied a novel single-biopsy and double-tracer ([2H2]glycine and [15N]glycine) approach based on evaluation of steady-state precursor incorporation in product. The correlations between the traditional (multiple-samples, one-tracer) and new (one-sample, double-tracer infusion) methods were analysed in erythrocytes by Passing–Bablok and Altman–Bland tests. Muscle glutathione absolute synthesis rate increased following bed rest from 5.5±1.1 to 11.0±1.5 mmol (kg wet tissue)−1 day−1 (mean ± s.e.m.; n =9; P =0.02) while glutathione concentration failed to change significantly. Bed rest induced vastus lateralis muscle atrophy, as assessed by pennation angle changes measured by ultrasonography (from 18.6±1.0 to 15.3±0.9 deg; P =0.01) and thickness changes (from 2.3±0.2 to 1.9±0.1 cm; P <0.001). Moreover, bed rest increased protein oxidative stress, as measured by muscle protein carbonylation changes (from 0.6±0.1 to 1.00±0.1 Oxydized-to-total protein ratio; P <0.04). In conclusion, we developed in erythrocytes a new minimally invasive method to determine peptide synthesis rate in human tissues. Application of the new method to skeletal muscle suggests that disuse atrophy is associated to oxidative stress induction aswell as to compensatory activation of the glutathione system.

Item URL in elib:https://elib.dlr.de/68481/
Document Type:Article
Title:Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Agostini, FrancescoUniversity of TriesteUNSPECIFIED
Dalla Libera, LucianoUniversity of PadovaUNSPECIFIED
Rittweger, Jörnjoern.rittweger (at) dlr.deUNSPECIFIED
Mazzucco, SaraUniversity of TriesteUNSPECIFIED
Jurdana, MihaelaUniversity of PrimorskaUNSPECIFIED
Mekjavic, IgorJozef Stefan InstituteUNSPECIFIED
Pisot, RadoUniversity of PrimorskaUNSPECIFIED
Gorza, LuisaUniversity of PadovaUNSPECIFIED
Narici, MarcoManchester Metropolitan UniversityUNSPECIFIED
Biolo, GianniUniversity of TriesteUNSPECIFIED
Date:2010
Journal or Publication Title:The Journal of Physiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:588
Page Range:pp. 5089-5104
Publisher:Wiley
ISSN:0022-3751
Status:Published
Keywords:Bed rest, muscle atrophy
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Rittweger, Jörn
Deposited On:09 Feb 2011 11:01
Last Modified:06 Sep 2019 15:30

Available Versions of this Item

  • Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach. (deposited 09 Feb 2011 11:01) [Currently Displayed]

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.