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A bacterial consortium attenuates a low-dose gamma irradiation effect in kalanchoe plantlets

Burlak, O. and Rogutskyy, I. and Mikheev, O. and Zaets, I. and Chervatyuk, N. and de Vera, Jean Pierre Paul and Danilchenko, B. and Foing, B. and Kоzyrovska, N. (2010) A bacterial consortium attenuates a low-dose gamma irradiation effect in kalanchoe plantlets. Kosmična nauka i technolohija: KNiT, 16, pp. 75-80.

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Abstract

The ability of plants to protect themselves against ionizing radiation is limited. One of the way how to alleviate irradiation consequences in plants is to use plant-associated bacteria for inoculation. Two defined plant growth promoting bacterial strains were used for inoculation Kalanchoe daigremontiana plantlets before acute irradiation with g-quanta (60Co). Lethal doses of the g-rays for Klebsiella oxytoca IMBG26 was 3,0 kGy, and for Paenibacillus sp. IMBG156 it was 500 Gy. Bacteria expressed enhanced the pelX promoter activity after sublethal dose irradiation. The pelX promoter activity that measured as activity of β-galactosidase of the pelX::lacZ fusion in K. oxytoca (pGalP) was 0,88 mkM/ml•min after exposure to 2,0 kGy, e.a. 80 % of control (untreated) bacterial activity, although the irradiated bacterial population comprised 1,25 % from control one. Integrated index of plantlets development which relied on both root number and root length reflected fluctuations in metabolic processes in irradiated plantlets without treatment with bacteria. Stabilizing stress-reactions occurred 10 days post irradiation at different doses (30, 50, 70 Gy), however, index of growth (IG) remained at the level of 30-60 % to control plantlets. The effect of irradiation on kalanchoe plantlets was relieved by bacteria at doses of 30 and 50 Gy. Moreover, IG was observed at levels of 500 and 200 %, respectively, and a 30 Gy dose was obviously stimulating for K. daigremontiana plantlets. Intense root elongation, instead of development of new coronal roots, led to fast adaptation to stressful conditions and normalization of metabolic processes in kalanchoe plantlets. However, integrated index showed inhibition of both inoculated and non-inoculated plantlets development after getting a 70 Gy dose.

Item URL in elib:https://elib.dlr.de/68135/
Document Type:Article
Title:A bacterial consortium attenuates a low-dose gamma irradiation effect in kalanchoe plantlets
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Burlak, O.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
Rogutskyy, I.Institute of Physics of NASU, UkraineUNSPECIFIED
Mikheev, O.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
Zaets, I.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
Chervatyuk, N.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Danilchenko, B.Institute of Physics of NASU, UkraineUNSPECIFIED
Foing, B.European Space Agency, ESTEC, Research and Scientific Support Department, Noordwijk, The NetherlandsUNSPECIFIED
Kоzyrovska, N.Institute of Molecular Biology&Genetics, National Academy of Sciences of Ukraine, KyivUNSPECIFIED
Date:2010
Journal or Publication Title:Kosmična nauka i technolohija: KNiT
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:16
Page Range:pp. 75-80
Status:Published
Keywords:gamma-irradiation, bacterial consortium, β-galactosidase, kalanchoe, stimulating effect, plant protection
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W EW - Erforschung des Weltraums
DLR - Research area:Space
DLR - Program:W EW - Erforschung des Weltraums
DLR - Research theme (Project):W - Vorhaben Planetenforschung (old)
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:10 Jan 2011 09:16
Last Modified:01 Dec 2018 19:47

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