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Horn, Astrid und Ullrich, Oliver und Huber, Kathrin und Hemmersbach, Ruth (2011) PMT (Photomultiplier) Clinostat. Microgravity Science and Technology, 23 (1), Seiten 67-71. Springer. doi: 10.1007/s12217-010-9234-5. ISSN 0938-0108 (online), 1875-0494 (Print).
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Offizielle URL: http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s12217-010-9234-5
Kurzfassung
In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute – rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation.
| elib-URL des Eintrags: | https://elib.dlr.de/63420/ | ||||||||||||||||||||
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| Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||
| Zusätzliche Informationen: | Published online: 15.09.2010 | ||||||||||||||||||||
| Titel: | PMT (Photomultiplier) Clinostat | ||||||||||||||||||||
| Autoren: |
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| Datum: | 1 Januar 2011 | ||||||||||||||||||||
| Erschienen in: | Microgravity Science and Technology | ||||||||||||||||||||
| Referierte Publikation: | Ja | ||||||||||||||||||||
| Open Access: | Nein | ||||||||||||||||||||
| Gold Open Access: | Nein | ||||||||||||||||||||
| In SCOPUS: | Ja | ||||||||||||||||||||
| In ISI Web of Science: | Ja | ||||||||||||||||||||
| Band: | 23 | ||||||||||||||||||||
| DOI: | 10.1007/s12217-010-9234-5 | ||||||||||||||||||||
| Seitenbereich: | Seiten 67-71 | ||||||||||||||||||||
| Verlag: | Springer | ||||||||||||||||||||
| ISSN: | 0938-0108 (online), 1875-0494 (Print) | ||||||||||||||||||||
| Status: | veröffentlicht | ||||||||||||||||||||
| Stichwörter: | Clinostat, Luminescence measurement, Oxidative burst, TRIPLE LUX | ||||||||||||||||||||
| HGF - Forschungsbereich: | Verkehr und Weltraum (alt) | ||||||||||||||||||||
| HGF - Programm: | Weltraum (alt) | ||||||||||||||||||||
| HGF - Programmthema: | W FR - Forschung unter Weltraumbedingungen (alt) | ||||||||||||||||||||
| DLR - Schwerpunkt: | Weltraum | ||||||||||||||||||||
| DLR - Forschungsgebiet: | W FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||
| DLR - Teilgebiet (Projekt, Vorhaben): | W - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt) | ||||||||||||||||||||
| Standort: | Köln-Porz | ||||||||||||||||||||
| Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Biomedizinisches Wissenschafts-Unterstützungszentrum | ||||||||||||||||||||
| Hinterlegt von: | Lütz, Gertrud | ||||||||||||||||||||
| Hinterlegt am: | 08 Mär 2010 09:56 | ||||||||||||||||||||
| Letzte Änderung: | 26 Mär 2013 13:15 |
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