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Horn, Astrid and Ullrich, Oliver and Huber, Kathrin and Hemmersbach, Ruth (2011) PMT (Photomultiplier) Clinostat. Microgravity Science and Technology, 23 (1), pp. 67-71. Springer. doi: 10.1007/s12217-010-9234-5. ISSN 0938-0108.
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Official URL: http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s12217-010-9234-5
Abstract
In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute – rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation.
| Item URL in elib: | https://elib.dlr.de/63420/ | ||||||||||||||||||||
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| Document Type: | Article | ||||||||||||||||||||
| Additional Information: | Published online: 15.09.2010 | ||||||||||||||||||||
| Title: | PMT (Photomultiplier) Clinostat | ||||||||||||||||||||
| Authors: |
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| Date: | 1 January 2011 | ||||||||||||||||||||
| Journal or Publication Title: | Microgravity Science and Technology | ||||||||||||||||||||
| Refereed publication: | Yes | ||||||||||||||||||||
| Open Access: | No | ||||||||||||||||||||
| Gold Open Access: | No | ||||||||||||||||||||
| In SCOPUS: | Yes | ||||||||||||||||||||
| In ISI Web of Science: | Yes | ||||||||||||||||||||
| Volume: | 23 | ||||||||||||||||||||
| DOI: | 10.1007/s12217-010-9234-5 | ||||||||||||||||||||
| Page Range: | pp. 67-71 | ||||||||||||||||||||
| Publisher: | Springer | ||||||||||||||||||||
| ISSN: | 0938-0108 | ||||||||||||||||||||
| Status: | Published | ||||||||||||||||||||
| Keywords: | Clinostat, Luminescence measurement, Oxidative burst, TRIPLE LUX | ||||||||||||||||||||
| HGF - Research field: | Aeronautics, Space and Transport (old) | ||||||||||||||||||||
| HGF - Program: | Space (old) | ||||||||||||||||||||
| HGF - Program Themes: | W FR - Forschung unter Weltraumbedingungen (old) | ||||||||||||||||||||
| DLR - Research area: | Space | ||||||||||||||||||||
| DLR - Program: | W FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||
| DLR - Research theme (Project): | W - Vorhaben Biowissenschaftliche Nutzerunterstützung (old) | ||||||||||||||||||||
| Location: | Köln-Porz | ||||||||||||||||||||
| Institutes and Institutions: | Institute of Aerospace Medicine > Biomedical Science Support Center | ||||||||||||||||||||
| Deposited By: | Lütz, Gertrud | ||||||||||||||||||||
| Deposited On: | 08 Mar 2010 09:56 | ||||||||||||||||||||
| Last Modified: | 21 Oct 2024 10:53 |
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