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DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis

Moeller, Ralf and Stackebrandt, Erko and Douki, Thierry and Cadet, Jean and Rettberg, Petra and Mollenkopf, Hans-Joachim and Reitz, Günther and Horneck, Gerda (2007) DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis. Archives of Microbiology, 188 (4), pp. 421-431. Springer. DOI: 10.1007/s00203-007-0263-4

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Abstract

Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.

Item URL in elib:https://elib.dlr.de/52964/
Document Type:Article
Title:DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Moeller, RalfGerman Collection of Microorganisms and Cell Cultures (DSMZ), Mascheroder Weg 1b, D-38124 Braunschweig, Germany, and German Aerospace Center (DLR), Radiation Biology DivisionUNSPECIFIED
Stackebrandt, ErkoGerman Collection of Microorganisms and Cell Cultures (DSMZ), Mascheroder Weg 1b, D-38124 Braunschweig, GermanyUNSPECIFIED
Douki, ThierryDépartement de Recherche Fondamentale sur la Matière Condensée CEA-Grenoble, Laboratoire “Lésions des AcidesNucléiques”, Service de Chimie Inorganique et Biologique,UMR-E3 (CEA/UJF), 38054 Grenoble Cedex 9, FranceUNSPECIFIED
Cadet, JeanDépartement de Recherche Fondamentale sur la Matière Condensée CEA-Grenoble, Laboratoire “Lésions des AcidesNucléiques”, Service de Chimie Inorganique et Biologique,UMR-E3 (CEA/UJF), 38054 Grenoble Cedex 9, FranceUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Mollenkopf, Hans-JoachimMax Planck Institute for Infection Biology (MPIIB),Microarray Core Facility, Berlin, GermanyUNSPECIFIED
Reitz, GüntherUNSPECIFIEDUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Date:2007
Journal or Publication Title:Archives of Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:188
DOI :10.1007/s00203-007-0263-4
Page Range:pp. 421-431
Publisher:Springer
Status:Published
Keywords:Bacillus subtilis, UV-C radiation, DNA repair, Gene expression, DNA photoproducts
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:21 Jan 2008
Last Modified:27 Apr 2009 14:40

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